巨藻中谷胱甘肽S转移酶基因对细长聚球藻PCC7942耐镉性的影响  

Effects of the Glutathione S-Transferase Gene Extracted from Giant Kelp(Macrocystis pyrifera) on the Cadmium Tolerance of Synechococcus elongatus PCC7942

作　　者：顾梓鹏 任玉东 程芬 张晓雯[3,4] 徐东 叶乃好[3,4] 梁成伟 GU Zipeng;REN Yudong;CHENG Fen;ZHANG Xiaowen;XU Dong;YE Naihao;LIANG Chengwei(College of Marine Science and Biological Engineering,Qingdao University of Science and Technology,Qingdao,Shandong 266042,China;Hainan Provincial Key Laboratory of Tropical Maricultural Technologies,Hainan Academy of Ocean and Fisheries Sciences,Haikou,Hainan 571126,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,Shandong 266071,China;Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao,Shandong 266071,China)

机构地区：[1]青岛科技大学海洋科学与生物工程学院,山东青岛266042 [2]海南省海洋与渔业科学院、海南省热带海水养殖技术重点实验室,海南海口571126 [3]中国水产科学研究院黄海水产研究所,山东青岛266071 [4]青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266071

出　　处：《渔业科学进展》2023年第2期127-136,共10页Progress in Fishery Sciences

基　　金：海南省热带海水养殖技术重点实验室开放基金(TMTOF202105);国家自然科学基金(31770393);泰山学者青年专家项目(tsqn202103136)共同资助。

摘　　要：谷胱甘肽S转移酶(glutathione S-transferase, GST)是一个较大的基因家族,在生物体生长发育和对环境变化响应中发挥重要的调控作用。本研究从巨藻(Macrocystis pyrifera)配子体中克隆了6个完整的GST基因(mpgst1、mpgst2、mpgst3、mpgst4、mpgst5和mpgst6)。随后将6个巨藻GST基因分别转化至细长聚球藻(SynechococcuselongatusPCC7942)中,提取细长聚球藻转化株基因组DNA作为模板进行PCR验证及测定转化株GST酶活进行基因功能验证,结果显示,6个mpgst基因都分别成功整合到细长聚球藻的基因组中,但只有含mpgst1、mpgst4和mpgst6基因的细长聚球藻转化株(MG1、MG4和MG6)具有耐镉性。在镉离子胁迫下,细长聚球藻转化株MG1、MG4和MG6的生长、光合色素含量和叶绿素荧光参数Fv/Fm值均显著高于野生株(P<0.05)。本研究结果为进一步研究巨藻GST基因的抗重金属胁迫功能奠定了基础。Giant kelp(Macrocystis pyrifera, Laminariales), has long been considered one of the most promising macroalgal species for biomass production because of its large size, rapid growth rate, and dynamic life history strategies. Brown seaweeds are economically important and commonly used for agricultural and industrial purposes. Intertidal and subtidal habitats, which most brown algae inhabit, are shaped by fluctuating levels of salinity-, temperature-, and light-related stresses. The responses of brown algae to abiotic stress have been comparatively well studied. With the rapid development of modern industry and agriculture as well as the exploitation of mineral resources, the pollution of ecological environments, particularly heavy-metal contamination of water, is becoming increasingly serious. Natural quantities of heavy metals in seawater do not adversely affect marine life, and some heavy metals even serve as trace nutrients essential for the normal growth and metabolism of algae. However, at excess concentrations, heavy metals act as pollutants and harm algae, and the magnitude of their impact varies depending on the degree of pollution. High metal concentrations negatively affect diatoms by inhibiting growth, triggering oxidative damage, modifying gene expression, damaging photosynthetic cells and mitochondria, and disrupting various cellular processes. Among the various metals, cadmium is particularly toxic and can easily accumulate in many marine organisms. Usually, cadmium concentrations in the sediment and open seawater are low, although these values may increase in some offshore and estuarine areas due to leakage or anthropogenic emissions. Glutathione S-transferase(GST) is a phase Ⅱenzyme in cells that catalyzes the formation of chelates between reduced glutathione and metal ions as well as the binding of sulfur atoms of reduced glutathione to phase Ⅰ electrophilic groups, thereby reducing the levels of intracellular toxic substances, such as reactive oxygen species, and accelerating their exogenous r

关 键 词：谷胱甘肽S转移酶基因 转基因 镉离子胁迫 巨藻 细长聚球藻PCC7942 

分 类 号：Q78[生物学—分子生物学]