高蛋氨酸饮食诱导大鼠H型高血压  

Methionine-richdiet induces hyperhomocysteine-associated hypertension in rats

作　　者：刘凌云 肖宗慧[1] 白宝玲[1] 张勤[1] LIU Lingyun;XIAO Zonghui;BAI Baoling;ZHANG Qin(Biochemistry and Immunology Research Unit,Capital Institute of Pediatrics,Beijing 100020,China)

机构地区：[1]首都儿科研究所生化免疫研究室,100020

出　　处：《国际心血管病杂志》2023年第2期104-107,共4页International Journal of Cardiovascular Disease

摘　　要：目的:观察高蛋氨酸饮食对大鼠血压和血同型半胱氨酸(Hcy)水平的影响。方法:8周龄雄性SD大鼠24只,随机分为3组:对照组、高蛋氨酸饮食组和高蛋氨酸饮食后叶酸回补组。对照组大鼠予普通饲料喂养16周,高蛋氨酸饮食组大鼠予蛋氨酸饲料(蛋氨酸为7.7 g/kg)喂养16周,高蛋氨酸饮食后叶酸回补组予蛋氨酸饲料喂养16周后,继续给予蛋氨酸叶酸饲料(蛋氨酸为7.7 g/kg,叶酸为100 mg/kg)喂养4周。每周记录各组大鼠的收缩压(SBP)。喂养结束后处死大鼠,检测血浆Hcy浓度。结果:实验结束时,对照组、高蛋氨酸饮食组和高蛋氨酸饮食后叶酸回补组的Hcy测值分别为4.03、6.16和4.91μmol/L,3组间比较差异有统计学意义(P=0.004)。喂养16周后,高蛋氨酸饮食组(149.8 mmHg)及高蛋氨酸饮食后叶酸回补组(150.0 mmHg)的SBP水平均高于对照组(120.0 mmHg)。叶酸喂养4周后高蛋氨酸饮食后叶酸回补组大鼠的血压降为115.5 mmHg,与对照组比较差异无统计学意义(P>0.05)。结论:高蛋氨酸饮食可诱导大鼠发生H型高血压,叶酸可能有一定的治疗作用。Objective:To explore the effect of methionine-rich diet on blood pressure andplasma homocysteine(Hcy)levels.Methods:Twenty male Sprague-Dawley rats(aged 8 weeks)were randomly divided into three groups as follows:control group,Hcy group and Hcy+folic acid group.Rats in the control group were fed with a regular diet and those in the Hcy group were fed with a methionine-rich diet(7.7g/kg L)for 16 weeks.Rats in the Hcy+folic acid group were fed with a methionine-rich diet for 16 weeks,followed by supplementary folic acid(100 mg/kg)for an additional four weeks.Body weight and systolic blood pressure were recorded weekly.All rats were sacreficed,and plasma Hcy levels were determined.Results:At the end of the experiment,plasma Hcy levels were 4.03,6.16 and 4.91μmol/L in control group,Hcy group and Hcy+folic acid group,respectively,with significant differences among the three groups(P=0.004).At 16 weeks,systolic blood pressure was higher in Hcy group(149.8 mmHg)and in Hcy+folic acid group(150.0 mmHg)compared to control group(120.0 mmHg).After an additional 4 weeks of folic acid feeding,systolic blood pressure decreased to 115.5 mmHg,which was not significantly different from that in control group(P>0.05).Conclusion:Methionine-rich diet induces Hcy-associated systolic hypertension in rats,and folic acid may have a therapeutic effect.

关 键 词：蛋氨酸 同型半胱氨酸 叶酸 H型高血压 

分 类 号：R544.11[医药卫生—心血管疾病]