正交试验优化半仿生提取刺梨果渣中黄酮的研究  

Optimization of Semi-Bionic Extraction of Flavonoids From the Rosa Roxburghii Tratt Pomace by Orthogonal Design

作　　者：袁爽 姚艳 石慧 汪祖华 陈滕 朱月 俸婷婷 Yuan Shuang;Yao Yan;Shi Hui;Wang Zuhua;Chen Teng;Zhu Yue;Feng Tingting(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Research Center forApplication and Development of Medicine and Food Dual-use Resources,Guizhou University of Traditional ChineseMedicine,Guiyang 550025,China;Nano-drug Technology Research Center of Guizhou University ofTraditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州中医药大学药食两用资源应用与开发研究中心,贵州贵阳550025 [3]贵州中医药大学纳米药物技术研究中心,贵州贵阳550025

出　　处：《山东化工》2023年第2期35-38,共4页Shandong Chemical Industry

基　　金：贵州省大学生创新创业训练计划项目(202110662060);贵州中医药大学大学生创新创业训练计划项目(贵中医大创合字(2021)86号);贵州中医药大学本科教学工程建设项目(贵中医教学工程合字(2019)54号)。

摘　　要：目的:优选半仿生法提取刺梨果渣黄酮的工艺条件。方法:采用单因素结合正交试验,考查乙醇体积分数、料液比、提取温度、提取时间对刺梨果渣黄酮提取率的影响。结果:半仿生法提取刺梨果渣中黄酮的最佳工艺条件为:乙醇体积分数60%、料液比1∶15(g∶mL)、提取温度80℃、提取时间分别是70,35,35 min,该工艺条件下刺梨果渣黄酮提取率为0.58%。结论:所建立的提取工艺操作简单、提取率高,可用于刺梨果渣中黄酮的提取,为综合开发刺梨加工副产物刺梨果渣提供了参考依据。Objective:To optimize the conditions of semi-bionic extraction method of flavonoids from Rosa roxburghii Tratt pomace.Methods:Single factor and orthogonal experiment were used to investigate the effects of ethanol volume fraction,solid-liquid ratio,extraction temperature and extraction time on the extraction rate of flavonoids from Rosa roxburghii Tratt pomace.Results:The optimum conditions of semi-bionic extraction of flavonoids from the Rosa roxburghii Tratt pomace were as follows:ethanol volume fraction 60%,solid-liquid ratio 1∶15(g∶mL),extraction temperature 80℃and extraction time 70,35,35 min.Under this condition,the extraction rate of flavonoids from the Rosa roxburghii Tratt pomace was 0.58%.Conclusion:The established method has simple operation and high extraction rate,and can be used to extract flavonoids from Rosa roxburghii Tratt fruit residue,which provides a reference for comprehensive development of Rosa roxburghii Tratt pomace.

关 键 词：刺梨果渣 黄酮 半仿生法 提取率 正交试验 

分 类 号：TQ641[化学工程—精细化工]