抗草甘膦转基因大豆与野大豆杂交F_(3)代表型性状分析  

Analysis on phenotypic traits of F_(3) hybrids between glyphosate-resistant transgenic soybean and wild soybean

作　　者：刘佳丽 刘金悦 纪雪勤 强胜[1] 宋小玲[1] LIU Jiali;LIU Jinyue;JI Xueqin;QIANG Sheng;SONG Xiaoling(College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学生命科学学院,江苏南京210095

出　　处：《植物资源与环境学报》2023年第2期56-64,共9页Journal of Plant Resources and Environment

基　　金：国家自然科学基金项目(32171656);国家转基因生物新品种培育重大专项(2016ZX08012005)。

摘　　要：为了评估转基因大豆向野大豆(Glycine soja Sieb.et Zucc.)基因漂移后可能产生的生态风险,选取抗草甘膦转基因大豆(携带外源基因cp4-epsps)与野大豆(来自内蒙古包头)杂交F_(2)代种皮颜色为黑色、褐绿色和褐黄色(与野大豆种皮颜色相近)的种子,对这些种子萌发产生的F_(3)代植株的19个定量性状和5个定性性状进行分析,在此基础上,将F_(3)代植株及其亲本进行聚类分析,并对不同组F_(3)代的定量和定性性状进行分析。结果显示:F_(3)代幼苗中抗性植株与非抗性植株的分离比符合“5∶1”的孟德尔遗传定律。定量性状中,结实率的变异系数最小(4.65%),其余18个性状的变异系数高于14%,其中,单株种子数的变异系数最大(55.68%)。不同种皮颜色F_(2)代种子萌发产生的F_(3)代植株均以强缠绕型占比最高;F_(3)代植株中,90.7%的果荚为弯镰形或弓形,且80.0%的果荚颜色为黑褐色;黑色种皮F_(2)代种子萌发产生的F_(3)代种皮颜色无性状分离,褐绿色和褐黄色种皮F_(2)代种子萌发产生的F_(3)代种皮颜色均出现性状分离;F_(3)代种子中,82.7%的种子有泥膜。聚类结果显示F_(3)代植株被分为3组。不同组F_(3)代中,Ⅰ组的多数性状最低,且株高等5个性状显著低于野大豆;Ⅱ组的9个性状最高,且单株结荚数等15个性状显著高于野大豆,仅株高显著低于野大豆;Ⅲ组的部分性状最高,且单株结荚数等12个性状显著高于野大豆,株高等3个性状显著低于野大豆。综合比较认为,Ⅱ组的竞争能力最强,Ⅰ组的竞争能力最弱。Ⅰ组的不缠绕型植株占比最高,而Ⅱ和Ⅲ组的强缠绕型植株占比最高。3组F_(3)代的种皮颜色均以黑色占比最高。综上所述,供试的不同组F_(3)代植株均能完成生活史并产生后代。若抗草甘膦转基因大豆的基因通过花粉漂移到野大豆上并成功杂交,则会产生不同适应性杂交后代,增加生态风险。In order to evaluate the potential ecological risk of gene flow from transgenic soybean to wild soybean(Glycine soja Sieb.et Zucc.),seeds of F_(2) hybrids between glyphosate-resistant transgenic soybean(carrying foreign gene cp4-epsps)and wild soybean(collected from Baotou of Inner Mongolia)with black,brown green,and brown yellow seed coats(similar to the seed coat color of wild soybean)were selected,and analysis on 19 quantitative traits and 5 qualitative traits of F_(3) generation plants germinated from these seeds was performed,on the basis,cluster analysis on F_(3) generation plants and their parents was conducted,and analysis on quantitative and qualitative traits of different groups of F_(3) generation was carried out.The results show that the segregation ratio of resistant plants and non-resistant plants of F_(3) generation seedlings conforms to the Mendel s law of“5∶1”.Among quantitative traits,the coefficient of variation of seed setting rate is the smallest(4.65%),those of the other 18 traits are higher than 14%,in which,that of seed number per plant is the highest(55.68%).Strong winding type accounts for the highest proportion in F_(3) generantion plants germinated from F_(2) generation seeds with different seed coat colors;among F_(3) generation plants,90.7%of the pods are bend sickle-shaped or bow,and 80.0%of the pods are black brown;the seed coat color of F_(3) generation germinated from F_(2) generation seeds with black seed coat has no trait segregation,while that of F_(3) generation germinated from F_(2) generation seeds with brown green and brown yellow seed coats all have trait segregation;among F_(3) generation seeds,82.7%of the seeds have seed-bloom.The cluster result shows that the F_(3) generation plants can be divided into 3 groups.Among different groups of F_(3) generation,most traits of groupⅠare the lowest,and 5 traits such as plant height are significantly lower than those of wild soybean;9 traits of groupⅡare the highest,and 15 traits such as pod number per plant are signifi

关 键 词：抗草甘膦转基因大豆 野大豆 杂交F_(3)代 表型性状 性状分离 

分 类 号：Q945.3[生物学—植物学] S565.1[农业科学—作物学]