葱莲地上部和地下部总生物碱提取物对植物病原菌的体外抑制效应  

In vitro inhibitory effects from above- and under-ground parts of total alkaloid extracts of Zephyranthes candida on phytopathogens

作　　者：乔思薇 姚静远 印敏[2,3] 王忠[2,3] 王奇志 冯煦[2,3] 陈雨[2,3] 徐曙[2,3] QIAO Siwei;YAO Jingyuan;YIN Min;WANG Zhong;WANG Qizhi;FENG Xu;CHEN Yu;XU Shu(Nanjing University of Traditional Chinese Medicine,Nanjing 210023,China;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen)Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Nanjing 210014,China;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen)Jiangsu Provincial Engineering Research Center for Ecological Planting and High Value Utilization of Traditional Chinese Medicine,Nanjing 210014,China)

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏省中国科学院植物研究所(南京中山植物园)江苏省植物资源研究与利用重点实验室,江苏南京210014 [3]江苏省中国科学院植物研究所(南京中山植物园)江苏省中药材生态种植与高值化利用工程研究中心,江苏南京210014

出　　处：《植物资源与环境学报》2023年第2期95-97,共3页Journal of Plant Resources and Environment

基　　金：江苏省中国科学院植物研究所自主科研重点项目(BM2018021-5)。

摘　　要：为了探究葱莲(Zephyranthes candida(Lindl.)Herb.)在生物农药上的应用价值,采用水浴加热浸提法提取野生葱莲地上部和地下部的总生物碱,并比较质量浓度500μg·mL^(-1)地上部和地下部总生物碱提取物对常见植物病原菌(包括12种植物病原真菌和4种植物病原细菌)的抑制率。结果表明:葱莲地上部和地下部总生物碱的提取率分别为18.241%和9.725%。葱莲地上部和地下部总生物碱提取物对稻瘟病菌(Magnaporthe oryzae)的抑制率均较高,分别为75.29%和82.62%;2种提取物对其余植物病原真菌和植物病原细菌的抑制率均较低,多在20%以下。并且,葱莲地上部和地下部总生物碱提取物对多数植物病原菌的抑制率无显著差异。研究结果显示:葱莲地上部和地下部总生物碱提取物具有防治稻瘟病的潜力,且地上部总生物碱提取物的使用成本更低。In order to explore the application value of Zephyranthes candida(Lindl.)Herb.in biopesticides,the total alkaloids from above-and under-ground parts of wild Z.candida were extracted by using a water-bath heating extraction method,and the inhibition rates of total alkaloid extracts from above-and under-ground parts with mass concentration of 500μg·mL^(-1)on common phytopathogens(containing 12 plant-pathogenic fungi and 4 plant-pathogenic bacteria)were compared.The results show that the extraction rates of total alkaloids from above-and under-ground parts of Z.candida are 18.241%and 9.725%,respectively.The inhibition rates of total alkaloid extracts from above-and under-ground parts of Z.candida on Magnaporthe oryzae are relatively high,which are 75.29%and 82.62%,respectively;the inhibition rates of the two extracts on the other plant-pathogenic fungi and plant-pathogenic bacteria are relatively low,which are mostly lower than 20%.In addition,there is no significant difference in inhibition rates of total alkaloid extracts from above-and under-ground parts of Z.candida on most phytopathogens.It is suggested that the total alkaloid extracts from above-and under-ground parts of Z.candida have the potential for preventing and controlling rice blast,and the usage cost of total alkaloid extracts from above-ground part is lower.

关 键 词：葱莲 总生物碱 植物病原菌 抑制率 生物农药 

分 类 号：Q946.88[生物学—植物学] S482.292[农业科学—农药学]