碳氮磷交互作用对高浓度氨氮鸡粪沼液培养小球藻的影响  

Interaction Effects of Carbon, Nitrogen and Phosphorus on Chlorella Cultivation of by Chicken Manure Biogas Slurry with High Ammonium Concentration

作　　者：贾侑臻 卢海凤[1,2,3] 董泰丽 孙颖财 李保明 童勤[1,2,3] 胡国娜 张东明 韩挺 JIA Youzhen;LU Haifeng;DONG Taili;SUN Yingcai;LI Baoming;TONG Qin;HU Guona;ZHANG Dongming;HAN Ting(College of Water Resources and Civil Engineering,China Agricultural University,Beijing 100083,China;Key Laboratory of Agricultural Engineering in Structure and Environment,Ministry of Agriculture and Rural Affairs of the PRC,Beijing 100083,China;Beijing Engineering Research Center for Livestock Poultry Healthy Environment,Beijing 100083,China;Shandong Minhe Biotechnology Co Ltd,Penglai 265600,China)

机构地区：[1]中国农业大学水利与土木工程学院,北京100083 [2]农业农村部设施农业工程重点实验室,北京100083 [3]北京市畜禽健康养殖环境工程技术中心,北京100083 [4]山东民和生物科技股份有限公司,山东蓬莱265600

出　　处：《中国沼气》2023年第2期29-38,共10页China Biogas

基　　金：企业横向委托课题。

摘　　要：为降低鸡粪沼液对环境污染的同时寻求资源的再生利用,以鸡粪沼液膜过滤出水作为小球藻培养基,研究了二氧化碳(CO_(2))体积浓度(0.03%~10%)及氮磷比(N/P=10~260)对小球藻生物量与色素累积,以及对氨氮、磷酸盐去除的影响。试验结果表明:在CO_(2)体积浓度为7.5%、N/P=80的条件下,小球藻干重最高可达3.38 g·L^(-1),叶绿素(Chlorophyl,Chl a+b)浓度为30.78 mg·L^(-1),氨氮去除率为68.6%。CO_(2)浓度对小球藻累积生物量的影响更大,培养20 d后,额外补偿CO_(2)的各处理组中磷酸盐去除率均>98%。研究为鸡粪沼液膜过滤出水培养微藻的工业化应用奠定了基础。In order to reduce the pollution of chicken manure biogas slurry to the environment and reuse resource,the wastewater from chicken manure anaerobic fermentation biogas slurry membrane filtration was used as a medium for Chlorella cultivation.The carbon dioxide volume percentage(CO_(2))(0.03%~10%)and nitrogen-to-phosphorus ratio(N/P=10~260)on the dry cell weight ad pigments concentration of Chlorella,and the ammonium and phosphate removal were investigated.Results show that with 7.5%CO_(2)and N/P=80,the dry cell weight and total chlorophyll(Chlorophyll,Chl a+b)concentration of Chlorella reached 3.38 g·L^(-1)and 30.78 mg·L^(-1),respectively.The ammonium removal reached 68.6%.The CO_(2)volume percentage has a greater impact on biomass accumulation,especially after 20 days’cultivation,with additional CO_(2)supplementation,phosphate removal in each group was>98%.This work provides the foundation for the industrial application of microalgae cultivation in chicken manure biogas slurry.

关 键 词：小球藻 氮磷比 二氧化碳 鸡粪沼液 

分 类 号：S216.4[农业科学—农业机械化工程] X712[农业科学—农业工程]