丝胶对STZ致损伤的INS-1细胞的保护作用  

Protective Effects of Sericin on STZ-induced Injury INS-1 Cells

作　　者：李金尧 韩思雨 李雨欣 易梦雅 陈志宏[1] LI Jin-yao;HAN Si-yu;LIYii-xin;YI Meng-ya;CHEN Zhi-hong(Department of Human Anatomy,Chengde Medical University,Chengde,067000,China)

机构地区：[1]承德医学院人体解剖学教研室,河北承德067000

出　　处：《承德医学院学报》2023年第2期91-95,共5页Journal of Chengde Medical University

基　　金：河北省“三三三人才工程”资助项目(A2016005063)。

摘　　要：目的 探讨丝胶对链脲佐菌素(STZ)致损伤大鼠胰岛素瘤细胞(INS-1细胞)的保护作用。方法 实验以体外培养的INS-1细胞为研究对象,随机分为5组:正常对照组(常规条件培养细胞)、模型组(培养基中含10mmol/L的STZ)、丝胶低、中、高浓度组(培养基中分别含10mmol/L的STZ,及150μg/mL、300μg/mL、600μg/mL的丝胶),每组细胞药物作用时间均为24h。蛋白印迹和实时荧光定量PCR法检测各组细胞BCL-2与P53蛋白及mRNA的表达情况。流式细胞术检测各组细胞的凋亡情况。结果 与正常对照组相比,在模型组中,INS-1细胞中BCL-2蛋白和mRNA的表达显著减少,P53蛋白和mRNA的表达显著增加,早期凋亡率显著升高(P<0.05)。与模型组相比,丝胶各组INS-1细胞BCL-2蛋白与mRNA的表达均显著增多,P53的蛋白与mRNA的表达均显著下降,早期凋亡率显著下降(P<0.05);3个丝胶组两两比较差异均有统计学意义(P<0.05)。结论 丝胶对STZ致损伤INS-1细胞发挥保护作用的机制与调控凋亡蛋白,抑制INS-1细胞凋亡有关。Objective To explore the protective efcts of sericin on serptototocin(STD)induced injury rats'INS-1 cells.Methods INS-1 cells were cultured in vitro and mandomly divided into five groups.Nomal control group(culure under conventional coditios),model group(0mmol/L STZ),low sricin group(STZ+150ug/mL sericin),medum senicin group(STZ+300μg/mL sericin),high scricin group(STZ+600μg/mL sericin),The cells in five groups were cultumred rspctively with comesponding dngs for 24h.The protein and mRNA epressias ofBCL-2 and P53 were dected by Westem boting and real time PCR.Cell apoptosis was detcted by flow cytometry.Results Compared with the nomal control group,theexpression ofBCL-2 prolein and mRNA of INS-1 cell in the model group were remarkably decrascd,while the expression of P53 proletin and mRNA were remarkably increased(P<0.05),as well as the earty aptosis rate was remarkably increased(P<0.05).Compared with the model group,the epressios of BCL2 protein and mRNA in 3 sericin goups were remarkably increased,while the expression ofP53 polein and mRNA were reomarkably docrased(P<0.05),and the carly apoptosis rate was remarkably decreased(P<0.05).Moreover,there were stistically remarkable dfferences among the 3 sericin groups(P<0.05).Conclusion The protectivce eficts of sericin o STZ/induced injuy INS-1 cells is relatad to the regulation of apoptotic proteins and ihibinin ofINS-1 cell apoptosis.

关 键 词：丝胶 糖尿病 INS-1细胞 凋亡 

分 类 号：R587.1[医药卫生—内分泌]