基于UPLC-Q-TOF MS的水栀子药材指纹图谱建立及其与栀子药材成分差异分析  

Construction of UPLC-Q-TOF MS Fingerprints for Gardeniae Grandiflorae and Analysis of Ingredient Differences between Gardenia Jasminoides Ellis and Gardenia Jasminoides Ellis Var.Grandiflora Nakai

作　　者：刘和平 巫登菊[1] 余孝云 郭伦 蔡信福 尚强 LIU Heping;WU Dengju;YU Xiaoyun;GUO Lun;CAI Xinfu;SHANG Qiang(Sichuan Guangda Pharmaceutical Co.,Ltd.,Chengdu 611930,China;Sichuan Pharmaceutical Resources Sub Center,National Engineering Research Center for Modernization of Traditional Chinese Medicine,Chengdu 610041,China;College of Pharmacy,Jinan University,Guangzhou 510632,China;Joincare Pharmaceutical Group Industry Co.,Ltd.,Shenzhen 518000,China;Industrialization of Antiviral of Engineering Research Center for Modernization of Traditional Chinese Medicine in Sichuan Province,Chengdu 611930,China)

机构地区：[1]四川光大制药有限公司,四川成都611930 [2]国家中药现代化技术研究中心川药资源分中心,四川成都610041 [3]暨南大学药学院,广东广州510632 [4]健康元药业集团股份有限公司,广东深圳518000 [5]四川省抗病毒中药产业化工程技术研究中心,四川成都611930

出　　处：《成都大学学报（自然科学版）》2023年第1期11-17,共7页Journal of Chengdu University（Natural Science Edition）

基　　金：四川省科技成果转化示范项目(23ZHSF0218)。

摘　　要：建立水栀子药材超高效液相—质谱联用指纹图谱,分析水栀子与栀子药材指纹图谱的差异.采用80%甲醇超声提取,超高效液相色谱法测定指纹图谱,结合质谱、保留指数和相关文献对部分色谱峰定性鉴别;并通过相似度评价和主成分分析水栀子与栀子药材所含主要指标性成分的差异.结果显示,水栀子指纹图谱共有24个共有峰,确定了其中的14个成分.水栀子与栀子药材相似度较为一致,主成分分析可以准确区分水栀子与栀子药材.所建立的指纹图谱测定方法可以用于水栀子与栀子药材的鉴别,研究结果为水栀子与栀子药材真伪品鉴定提供了实验依据.This paper aims to establish UPLC-Q-TOF MS chromatographic fingerprints of fructus Gardenia jasminoides Ellis var.grandiflora Nakai,and to analyze the fingerprints differences between Gardenia jasminoides Ellis var.grandiflora Nakai.and Gardenia jasminoides Ellis.The sample was extracted by ultrasonic with 80%methanol,and the fingerprint was determined by ultra performance liquid chromatography.Some chromatographic peaks were qualitatively identified by MS,retention index and related literature;meanwhile,the main target components differences between Gardenia jasminoides Ellis var.grandiflora Nakai.and Gardenia jasminoides Ellis were analyzed by similarity evaluation system and principal component analysis.The results show that there are 24 common components in the fingerprint of Gardenia jasminoides Ellis var.grandiflora Nakai.and 14 of them are identified.The similarity is relatively consistent,but the sources of fructus Gardenia jasminoides Ellis var.grandiflora Nakai.and Gardenia jasminoides Ellis.can be distinguished accurately by principal component analysis.The established fingerprint method can be used for the identification of the source of Gardenia jasminoides Ellis var.grandiflora Nakai.and Gardenia jasminoides Ellis.The research results can provide experimental evidence for the identification of the authenticity of Gardenia jasminoides Ellis var.grandiflora Nakai.and Gardenia jasminoides Ellis.

关 键 词：水栀子 栀子 指纹图谱 液相色谱 相似度 主成分分析 

分 类 号：R284.1[医药卫生—中药学]