球茎甘蓝MYB115转录因子的克隆与表达分析  

Cloning and expression analysis of MYB115 transcription factor from Brassica oleracea L.var.caulorapa

作　　者：赵文菊 王紫莹 王鑫淼 巴桑措姆 李江[1,3] 任延靖 ZHAO Wenju;WANG Ziying;WANG Xinmiao;Basangcuomu;LI Jiang;REN Yanjing(Academy of Agriculture and Forestry Sciences,Key Laboratory of Vegetable Genetics and Physiology of Qinghai,Qinghai University,Xining 810016,China;Comprehensive Agriculture and Animal Husbandry Service Center of Yushu Tibetan Autonomous Prefecture,Yushu 815000,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农林科学院,青海省蔬菜遗传与生理重点实验室,青海西宁810016 [2]玉树藏族自治州农牧业综合服务中心,青海玉树815000 [3]青海大学三江源生态和高原农牧业国家重点实验室,青海西宁810016

出　　处：《青海大学学报》2023年第1期21-27,共7页Journal of Qinghai University

基　　金：国家大宗蔬菜产业技术体系项目(CARS-23-G26);青海省科学技术厅项目(2021-NK-124);青海省蔬菜遗传与生理重点实验室项目(2020-ZJ-Y02)。

摘　　要：为了深入挖掘MYB115转录因子的生物学功能,本研究以球茎甘蓝为试验材料,采用基因克隆获得球茎甘蓝MYB115转录因子,并对其进行生物信息学和荧光定量分析。生物信息学分析结果显示,BocMYB115转录因子gDNA序列全长742 bp,开放阅读框(ORF)长度为549 bp,编码182个氨基酸,具有一个高度保守的SANT结构域,属于1R-MYB类转录因子,与甘蓝型油菜的MYB115-like亲缘关系最近。荧光定量PCR结果显示,BocMYB115在绿色球茎甘蓝中的表达量显著高于紫色球茎甘蓝。本研究可为后续MYB115转录因子功能鉴定提供理论依据。Brassica oleracea L.var.caulorapa is taken as the material to further explore the biological functions of MYB115 transcription factor.Its MYB115 transcription factor is obtained by gene cloning technology.Its bioinformatics and fluorescence quantitative analysis are carried out as well.The results of bioinformatics analysis show that BocMYB115 gene sequence is 742 bp in length.ORF is 549 bp in length,encoding 182 amino acids.It has a highly conserved SANT domain belonging to 1R-MYB transcription factor,which is closely related to MYB115-like in Brassica napus.Accor-ding to the results of real-time PCR,the expression of BocMYB115 in green Brassica oleracea L.var.caulorapa is significantly higher than that in purple ones.This study lays a foundation for the functional identification of MYB115 transcription factor.

关 键 词：球茎甘蓝 MYB115 序列分析 保守基序 亲缘关系 荧光定量PCR 

分 类 号：S635.2[农业科学—蔬菜学]