HPLC法测定越鞠保和丸中苍术素、木香烃内酯和去氢木香内酯的含量  

Determination of atractylodin,costunolide and dehydrocostuslactone in Yuejubaohe pills by HPLC

作　　者：安劼 牛辰瑾 曲佳 申琳[3] An Jie;Niu Chenjin;Qu Jia;Shen Lin(Wuqing Branch of Tianjin Institute of Drug Control,Tianjin 301700;Tianjin Institute for Drug Control,Tianjin 300070;Second Teaching Hospital of Tjanjin University of Traditional Chinese Medcine,Tianjin 300250)

机构地区：[1]天津市药品检验研究院武清药品检验所,天津301700 [2]天津市药品检验研究院,天津300070 [3]天津中医药大学第二附属医院,天津300250

出　　处：《天津药学》2023年第1期17-21,共5页Tianjin Pharmacy

基　　金：国家中医药管理局全国中药特色技术传承人才项目(No.T20194828003)。

摘　　要：目的:建立HPLC法测定越鞠保和丸中苍术素、木香烃内酯和去氢木香内酯的含量。方法:使用C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇作为流动相A,0.2%磷酸水溶液作为流动相B,梯度洗脱;检测波长为木香烃内酯、去氢木香内酯225 nm,苍术素340 nm;柱温40℃;流速1.0 ml/min;进样量为10μl。结果:苍术素在1.036 79~103.679μg/ml范围内线性良好(r=1.000 0),木香烃内酯在2.951 17~118.046 8μg/ml范围内线性良好(r=1.000 0),去氢木香内酯在3.034~303.4μg/ml范围内线性良好(r=1.000 0)。苍术素平均回收率为103.20%,RSD为0.84%;木香烃内酯平均回收率为103.06%,RSD为0.52%;去氢木香内酯平均回收率为91.80%,RSD为0.72%。结论:本方法操作简单,专属性强,可以用于越鞠保和丸中苍术与木香两味药材的质量控制。Objective:To establish an HPLC method for the determination of atractylodin,costunolide and dehydrocostuslactone in Yuejubaohe pills.Methods:C18(250 mm×4.6 mm,5μm)chromatographic column was used,with methanol as mobile phase A and 0.2%phosphoric acid aqueous solution as mobile phase B,gradient elution;Detection wavelengths for costunolide and dehydrocostuslactone was 225 nm,for atractylodin was 340 nm;Column temperature was 40℃;Flow rate was 1 ml/min;Injection volume was 10μl.Results:The linearity was good in the range of 1.03679~103.679μg/ml for atractylodin(r=1.0000),2.95117~118.0468μg/ml for costunolide(r=1.0000)and 3.034~303.4μg/ml for dehydrocostuslactone(r=1.0000).The average recovery rate of atractylodin was 103.20%with the RSD was 0.84%;the average recovery rate of costunolide was 103.06%with the RSD was 0.52%;the average recovery rate of dehydrocostuslactone was 91.80%with the RSD was 0.72%.Conclusion:This method is simple to operate and has strong specificity,can be used for the quality control of atractylodes and aucklandiae radix in Yuejubaohe Pills.

关 键 词：越鞠保和丸 苍术素 木香烃内酯 去氢木香内酯 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]