过氧化氢酶BcCAT2在灰葡萄孢生长发育和致病过程中的功能初探  

Preliminary study on the function of catalase BcCAT2 in the development and pathogenesis of Botrytis cinerea

作　　者：宋美昕 张玮煜 王欣凯 张虎[1] 宋凯璇 邢继红[1] 张康 董金皋[1] SONG Meixin;ZHANG Weiyu;WANG Xinkai;ZHANG Hu;SONG Kaixuan;XING Jihong;ZHANG Kang;DONG Jingao(North China State Key Laboratory of Crop Improvement and Regulation/Hebei Provincial Key Laboratory of Plant Physiology and Molecular Pathology,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,河北保定071000

出　　处：《河北农业大学学报》2023年第2期16-21,共6页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31972217、32072369);中央引导地方科技发展基金项目(206Z6501G,216Z6502G);河北省自然科学基金项目(C2022204040);大中学生科技创新能力培育专项(22E50045D)。

摘　　要：为了明确灰葡萄孢过氧化氢酶BcCAT2在病菌生长发育时期和侵染过程中的功能,本研究利用生物信息学方法,对BcCAT2及其编码蛋白进行分析;构建了BcCAT2敲除载体,利用原生质体转化方法,获得了BcCAT2敲除突变体;利用PCR和qRT-PCR技术,对所获得的转化子进行鉴定;对野生型菌株和△Bccat2的表型(菌落形态、菌丝形态、生长速度、产孢量等)和致病力进行分析。结果发现,BcCAT2基因的缺失会导致菌丝形态纤细、生长速度缓慢、几乎不产菌核和分生孢子,且致病力明显下降,表明BcCAT2基因正调控病菌的生长发育和致病力。研究结果为进一步阐明灰葡萄孢过氧化氢酶BcCAT2基因调控病菌生长发育和致病力的分子机制奠定了基础。In order to clarify the function of catalase BcCAT2 in the growth and development stage and infection process of Botrytis cinerea,this study used bioinfonnatics methods to analyze BcCAT2 and its coding protein.BcCAT2 knockout vector was constructed to create BcCAT2 knockout mutant by protoplast transformation.PCR and qRT-PCR were used to identify the transformants.The phenotypes(colony morphology,mycelium morphology,growth rate,spore yield,etc.)and pathogenicity of the mutant h.Bccat2 and wild type strains were analyzed.The results showed that the deletion of BcCAT2 gene resulted in thin mycelia,slow growth,few sclerotia and conidia and decreased pathogenicity,indicating that BcCAT2 gene regulate the growth,development and pathogenicity of bacteria.The results lay a foundation for further elucidating the molecular mechanism of Botrytis cinerea BcCAT2 gene regulating the growth,development and pathogenicity of the pathogen.

关 键 词：灰葡萄孢 BcCAT2 生长发育 致病力 

分 类 号：S432.44[农业科学—植物病理学]