鼠李糖乳杆菌GR-1介导TRIF/NF-κB信号通路抗大肠杆菌诱导奶牛子宫内膜上皮细胞炎性损伤  

Protective effect of TRIF/NF-κB signal pathway mediated by Lactobacillus rhamnosus GR-1 on inflammatory damage of bovine endometrial epithelial cells induced by Escherichia coll

作　　者：冯晓微 刘佳玮 孙艳[1] 冯鸣鹊 靳天雄 刘蓓 程佳 刘明超 FENG Xiaowei;LIU Jiawei;SUN Yan;FENG Mingque;JIN Tianxiong;LIU Bei;CHENG Jia;LIU Mingchao(College of Veterinary Medicine,Hebei Agricultural University,Baoding 071001,China;Veterinary Biological Technology Innovation Center of Hebei Province,Baoding 071001,China)

机构地区：[1]河北农业大学动物医学院,河北保定071001 [2]河北省兽医生物技术创新中心,河北保定071001

出　　处：《河北农业大学学报》2023年第2期88-95,共8页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(31902328);河北省自然科学基金(C2021204067);河北省省属高等学校基本科研业务费研究项目(KY2021008);河北农业大学引进人才科研专项(ZD201723)。

摘　　要：为探讨鼠李糖乳杆菌GR-1(Lactobacillus rhamnosus GR-1,L.rhamnosus GR-1)对大肠杆菌(Escherichia coli)诱导的奶牛子宫内膜上皮细胞(Bovineendometrialepithelialcells,BEECs)炎性损伤的保护作用及其机制,本研究建立BEECs细胞炎性损伤模型,利用Real-time qPCR和Western blot技术,观察在BAY11-7082或siRNA干预前后,L.rhamnosus GR-1对E.coli触发的信号通路及炎性因子表达的影响。结果显示,L.rhamnosusGR-1可以显著抑制由E.coli引起的NF-κB和TRIF通路的激活及炎性因子的表达,在BAY11-7082干预后,E.coli诱导的NF-κB通路的激活及炎性因子的表达得到了显著的抑制。同样,在添加TRIF siRNA后,E.coli诱导的TRIF mRNA及炎性因子的表达水平也显著下降,产生和L.rhamnosus GR-1一致的抗炎效果。本研究表明L.rhamnosus GR-1可通过介导NF-κB和TRIF通路调节E.coli诱导的BEECs的炎性损伤,为奶牛子宫内膜炎的防治提供有益参考。The purpose of this study was to investigate the protective effect and mechanism of Lactobacillus rhamnosus GR-1(L.rhamnosus GR-1)on inflammatory injury of bovine endometrial epithelial cells(BEECs)induced by E.coli.In this study,the model of inflammatory injury of BEECs was established.Real-time qPCR and western blot were used to observe the effects of L.rhamnosus GR-1 on the signal pathway triggered by E.coli and the expression of inflammatory cytokines before and after the intervention of BAY11-7082 or siRNA.The results showed that L.rhamnosus GR-1 significantly inhibited the activation of NF-kB and TRIF pathways and the expression of inflammatory cytokines was induced by E.coli.After the intervention of BAY 11-7082,the NF-kB pathway was activated by E.coli and the expression of inflammatory cytokines were significantly inhibited.Similarly,after the addition of TRIF siRNA,the mRNA expression of TRIF that was induced by E.coli decreased significantly resulting in an anti-inflammatory effect which was consistent to that of L.rhamnosus GR-1.This study showed that L.rhamnosus GR-1 can reduce the inflammatory damage of BEECs induced by E.coli by mediating NF-kB and TRIF pathways,which provided a useful reference for the prevention and treatment of endometritis in dairy cows.

关 键 词：奶牛子宫内膜上皮细胞 鼠李糖乳杆菌 大肠杆菌 炎性反应 核转录因子ΚB β-干扰素TIR结构域衔接蛋白 

分 类 号：S854.5[农业科学—临床兽医学]