过氧化物酶基因TaPRX-2A调控小麦穗部性状的功能分析  

Function Analysis of TaPRX-2A Encoding Peroxidase on Regulating Traits of Wheat Spike

作　　者：苏培森 隋超 郭尚敬[1] 臧娇娇 满全财 SU Peisen;SUI Chao;GUO Shangjing;ZANG Jiaojiao;MAN Quancai(College of Agronomy and Agricultural Engineering,Liaocheng University,Liaocheng,Shandong 252000,China)

机构地区：[1]聊城大学农学与农业工程学院,山东聊城252000

出　　处：《麦类作物学报》2023年第3期296-301,共6页Journal of Triticeae Crops

基　　金：聊城大学博士科研启动基金项目(318052018);山东农业大学作物生物学国家重点实验室开放课题项目(2021KF03)。

摘　　要：为研究过氧化物酶基因TaPRX-2A在调控小麦穗部性状中的功能,以苏麦3号为材料,通过Ensemblplants数据库获得TaPRX-2A全长cDNA序列,利用PCR方法克隆获得该基因编码区全长序列。生物信息学分析结果表明,该基因包含一个1026 bp的开放阅读框,编码342个氨基酸。进一步构建基因过表达载体TaPRX-2A-PC186,通过基因枪介导的遗传转化方法将TaPRX-2A-PC186转入小麦KN199中,创建过表达TaPRX-2A转基因株系,调查TaPRX-2A基因对转基因株系穗部性状的影响。结果表明,过表达TaPRX-2A转基因株系的穗长显著变短,穗粒数显著减少,说明TaPRX-2A在调控小麦穗部发育过程中具有重要的作用。In order to study the function of peroxidase gene TaPRX-2A in regulating the spike traits of wheat,in this study,the cDNA sequence of TaPRX-2A was obtained from Ensembl plants database,and cloned by PCR method from the wheat variety Sumai 3.Bioinformatics analysis results showed that TaPRX-2A contained a 1026 bp ORF(open reading frame),encoding a total of 342 amino acids.The recombinant expression vector TaPRX-2A-PC186 was further constructed,and then transformed into KN199,to create TaPRX-2A transgenic overexpression lines.The effect of TaPRX-2A on the spike traits length of transgenic lines was studied.The results showed transgenic plants overexpressing TaPRX-2A had shorter spike length and fewer kernel number per spike,suggesting that TaPRX-2A played an important role in regulating the development of wheat spike.

关 键 词：小麦 TaPRX-2A基因 过表达 穗部性状 

分 类 号：S512.1[农业科学—作物学] S330