孕妇早期血清miR-29a-3p和VEGFA表达水平及其对子痫前期预测价值研究  

Study on the Expression Level of Serum miR-29a-3p and VEGFA in Early Pregnancy and Its Predictive Value for Preeclampsia

作　　者：康会贤 张蕾 马夕锐 崔照领 KANG Hui-xian;ZHANG Lei;MA Xi-rui;CUI Zhao-ling(the Seventh Department of Obstetrics,Shijiazhuang Maternal and Child Health Hospital,Shijiazhuang 050000,China;Department of Obstetrics and Gynecology,Shijiazhuang Maternal and Child Health Hospital,Shijiazhuang 050000,China)

机构地区：[1]石家庄市妇幼保健院产七科,石家庄050000 [2]石家庄市妇幼保健院妇产科,石家庄050000

出　　处：《现代检验医学杂志》2023年第2期32-36,共5页Journal of Modern Laboratory Medicine

基　　金：河北省医学科学研究重点课题(NO.20200145)。

摘　　要：目的研究微小核糖核酸(micro RNA,miR)-29a-3p和血管内皮生长因子A(vascular endothelial growth factor A,VEGFA)在子痫前期患者血清中的表达及意义。方法前瞻性选取2018年1月~2021年1月在石家庄市妇幼保健院行产检的600例孕妇为研究对象,对孕妇随访至分娩结束,根据孕妇是否患子痫前期,分为子痫前期组(n=60)和非子痫前期组(n=540)。实时荧光定量PCR法检测在孕10~14周入院产检时血清中miR-29a-3p的水平,采用酶联免疫吸附法(ELISA)测定血清VEGFA水平;采用Pearson法分析miR-29a-3p水平与VEGFA的相关性。采用受试者工作特征(ROC)曲线评价血清miR-29a-3p和VEGFA以及二者联合检测预测子痫前期的价值。结果子痫前期组miR-29a-3p(2.05±0.54)水平高于非子痫前期组(1.04±0.29),VEGFA水平(34.38±9.25 ng/L)低于非子痫前期组(69.49±19.57 ng/L),差异具有统计学意义(t=22.951,13.824,均P<0.05)。重度组血清中VEGFA水平(24.05±6.41 ng/L)显著低于轻度组(38.81±10.47 ng/L),miR-29a-3p(2.45±0.61)水平显著高于轻度组(1.88±0.51),差异均有统计学意义(t=5.537,3.738,均P<0.05)。Pearson分析显示,子痫前期患者血清中miR-29a-3p与VEGFA呈负相关(r=-0.601,P<0.05)。ROC曲线显示,血清中miR-29a-3p和VEGFA水平预测子痫前期的曲线下面积(AUC)分别为0.904,0.882;两者联合预测子痫前期的AUC为0.937,其灵敏度和特异度分别为80.00%,95.20%。结论子痫前期患者血清中miR-29a-3p表达上升,而VEGFA表达下降,血清miR-29a-3p和VEGFA对子痫前期具有一定的预测价值。Objective To study the expression and significance of microRNA(miR)-29a-3p and vascular endothelial growth factor A(VEGFA)in the serum of patients with preeclampsia.Methods Prospectively selected of 600 pregnant women undergoing maternity check-ups in Shijiazhuang Maternal and Child Health Hospital from January 2018 to January 2021 were selected as the research objects.The pregnant women were followed up to the end of delivery,according to whether the pregnant women had preeclampsia,they were divided into preeclampsia group(n=60)and non-preeclampsia group(n=540).The level of miR-29a-3p in serum was detected by real-time fluorescence quantitative PCR method at the 10th to 14th week of gestation at the time of admission for obstetric examination.Enzyme-linked immunosorbent assay(ELISA)was used to determine the level of serum VEGFA,Pearson method was used to analyze the correlation between miR-29a-3p level and VEGFA,and receiver operating characteristic(ROC)curve was used to evaluate the value of serum miR-29a-3p,VEGFA,and the combined detection in predicting preeclampsia.Results MiR-29a-3p level(2.05±0.54)in the preeclampsia group were higher than those in the non-preeclampsia group(1.04±0.29),the level of VEGFA(34.38±9.25 ng/L)was lower than that of the non-preeclampsia group(69.49±19.57 ng/L),and the differences were statistically significant(t=22.951,13.724,all P<0.05).The level of serum VEGFA in the severe group(24.05±6.41 ng/L)was significantly lower than that in the mild group(38.81±10.47 ng/L),and the level of miR-29a-3p(2.45±0.61)was significantly higher than that of the mild group(1.88±0.51),and the differences were statistically significant(t=5.537,3.738,all P<0.05).Pearson analysis showed that the level of serum miR-29a-3p in patients with preeclampsia was negatively correlated with VEGFA(r=-0.601,P<0.05).The ROC curve showed that the area under the curve(AUC)of serum miR-29a-3p and VEGFA levels predicting preeclampsia was 0.904 and 0.882,respectively,The AUC of the two combined prediction of pre

关 键 词：子痫前期 微小核糖核酸-29a-3p 血管内皮生长因子A 

分 类 号：R714.244[医药卫生—妇产科学] R392.11[医药卫生—临床医学]