二氧化锰纳米颗粒调控的紫外可见吸收-荧光双通道传感器的构建及D-青霉胺的检测  

Construction of MnO_(2) nanoparticles mediated UV-visible absorption-fluorescence dual channel sensor and detection of D-penicillamine

作　　者：程莹 柳夷冰 赵卉垚 南鑫达 范小青 李生玲 丁莉峰 王琦 牛宇岚 CHENG Ying;LIU Yi‑Bing;ZHAO Hui‑Yao;NAN Xin‑Da;FAN Xiao‑Qing;LI Sheng‑Ling;DING Li‑Feng;WANG Qi;NIU Yu‑Lan(Chemistry&Chemical Engineering Department,Taiyuan Institute of Technology,Taiyuan 030008,China;Zhejiang Province Quzhou City Longyou Country Inspection and Testing Institute,Quzhou,Zhejiang 324400,China)

机构地区：[1]太原工业学院化学与化工系,太原030008 [2]浙江省衢州市龙游县检验检测研究院,衢州324400

出　　处：《无机化学学报》2023年第4期617-626,共10页Chinese Journal of Inorganic Chemistry

基　　金：国家级大学生创新创业训练项目(No.202214101003);太原工业学院青年学术带头人支持计划(No.2020XS02);山西省1331项目资助。

摘　　要：以聚乙烯亚胺(PEI)同时作为模板和还原剂,基于原位氧化还原反应,一步法制备二氧化锰纳米颗粒(MnO_(2)NPs),并对其形貌、组成、紫外可见吸收、催化氧化特性进行了表征。结果表明,MnO_(2)NPs能够催化氧化邻苯二胺(OPD)为2,3-二氨基吩嗪(DAP),产生420 nm处的紫外可见吸收和560 nm处的荧光发射。D-青霉胺(DPA)中的活性巯基可与MnO_(2)发生特异性反应,还原降解催化剂,抑制其催化氧化活性,使得体系紫外可见吸收-荧光信号减弱甚至消失。基于DPA浓度与体系光谱信号变化的关系,我们建立了MnO_(2)NPs介导的紫外可见吸收-荧光双通道传感DPA的新方法。荧光传感通道具有更好的线性范围和灵敏度,该方法线性范围为1~80μmol·L^(-1),检出限为0.54μmol·L^(-1)。此外,MnO_(2)NPs介导的荧光传感DPA应用于人体尿液样品分析中的回收率为98.31%~107.76%,证明了该方法的可靠性。In this work,manganese dioxide nanoparticles(MnO_(2) NPs)were synthesized through a in situ redox reaction using polyethyleneimine(PEI)as both template and reductant.The morphology,composition,UV‑visible absorption,and catalytic oxidation performance of MnO_(2) NPs were characterized.MnO_(2) NPs possessed the characteristic of catalytic oxidation of o‑phenylenediamine(OPD)to 2,3‑diaminophenazine(DAP),producing the UV‑visible absorption at 420 nm and fluorescence emission at 560 nm.Owing to its active sulfhydryl,D‑penicillamine(DPA)could decompose MnO_(2) NPs through a unique redox reaction.Therefore,the catalytic oxidation activity was inhibited that made UV‑visible absorption‑fluorescence signals decreased and even disappeared.Based on the relationship between spectral variation and DPA concentration,a MnO_(2) NPs mediated UV‑visible absorption‑fluorescence dual channel sensing method for DPA was established.Fluorescence channel sensing possessed satisfactory linear range and sensitivity.The linear range was 1‑80μmol·L^(-1) with a detection limit of 0.54μmol·L^(-1).Moreover,MnO_(2) NPs mediated fluorescence sensing was applied in human urine sample detection and the recovery was in a range of 98.31%‑107.76%,which proved the reliability of this method.

关 键 词：二氧化锰纳米颗粒 紫外可见 荧光 D-青霉胺 

分 类 号：O614.7[理学—无机化学] O657.3[理学—化学]