外源柠檬酸缓解高羊茅盐胁迫的机制  

Mechanism of exogenous citric acid alleviating salt stress in Festuca arundinacea

作　　者：陈瑶 凌宇 龙江宇 范吉标 CHEN Yao;LING Yu;LONG Jiangyu;FAN Jibiao(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,Jiangsu,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《草业科学》2023年第2期511-520,共10页Pratacultural Science

基　　金：江苏省林业科技创新与推广项目(No.LYKJ[2021]09)。

摘　　要：柠檬酸作为一种天然化合物,已被用作外源添加物来缓解植物所受的多种胁迫。本研究以高羊茅(Festuca arundinacea)为试验材料,设置两个盐浓度(150与300 mmol·L^(-1)),探究外源柠檬酸对不同浓度NaCl胁迫下高羊茅的生长生理、相关基因表达的影响。结果表明,在150 mmol·L^(-1)NaCl浓度下添加柠檬酸后,高羊茅的株高、叶片含水量及过氧化物酶等抗氧化酶的活性均有增加。此外,柠檬酸还通过促进叶绿素合成等改善了光合作用,并且诱导了SOS1、NHX1、ATCS的表达,进而调节了钠离子等造成的负面影响。而在300 mmol·L^(-1)盐浓度的长时间处理下,柠檬酸对高羊茅的生长生理等的调节效果不明显。这表明,当NaCl浓度≤300 mmol·L^(-1)时,添加柠檬酸可以有效缓解高羊茅受到的盐害。As a natural compound,citric acid has been used as an exogenous additive to alleviate environmental stresses on plants.In this study,tall fescue(Festuca arundinacea)was treated with two salt concentrations(150 and 300 mmol·L^(-1))to explore the effects of exogenous citric acid on the growth,physiological characteristics,and related gene expression levels of tall fescue under different concentrations of NaCl.The results showed that plant height,leaf water content,and the activities of antioxidant enzymes,such as peroxidase,were increased after the application of citric acid after 150 mmol·L^(-1) NaCl treatment.In addition,citric acid improved the photosynthesis of tall fescue by promoting chlorophyll synthesis.It also induced the expression of SOS1,NHX1,ATCS,and regulated the negative effects of Na+.However,under long-term treatment with 300 mmol·L^(-1) NaCl,citric acid had no significant effects on the growth and physiological conditions of tall fescue.These results indicated that,when the NaCl concentration was less than 300 mmol·L^(-1) or when plants were treated with 300 mmol·L^(-1) for a short time,the addition of citric acid effectively alleviated salt damage in tall fescue.

关 键 词：柠檬酸 盐胁迫 高羊茅 株高 叶片含水量 抗氧化酶 基因表达 

分 类 号：S688.4[农业科学—观赏园艺]