型变过程中亚洲小车蝗体内保幼激素滴度及其代谢相关基因表达量的变化  

Changes in the titer of juvenile hormone and expression levels of its metabolism-related genes in Oedaleus asiaticus(Orthoptera:Oedipodidae)during phase transition

作　　者：孙彦楠 闫锋 高书晶[1] 韩海斌[1] 郭娜 高海燕 杨旭兵 SUN Yan-Nan;YAN Feng;GAO Shu-Jing;HAN Hai-Bin;GUO Na;GAO Hai-Yan;YANG Xu-Bing(Institute of Grassland Research,Chinese Academy of Agricultural Sciences,Hohhot 010010,China;Ordos Forestry and Grassland Development Center,Ordos 017010,China)

机构地区：[1]中国农业科学院草原研究所,呼和浩特010010 [2]鄂尔多斯市林业和草原事业发展中心,鄂尔多斯017010

出　　处：《昆虫学报》2023年第2期130-137,共8页Acta Entomologica Sinica

基　　金：内蒙古自治区自然科学基金项目(2021MS03021);国家科技基础资源调查专项(2019FY100400);中央级公益性科研院所基本科研业务费专项资金(1610332020008)。

摘　　要：【目的】本研究旨在明确亚洲小车蝗Oedaleus asiaticus在不同发育阶段和型变过程中的保幼激素(juvenile hormone,JH)JHⅢ滴度及其代谢相关基因表达量的变化情况,为阐明保幼激素调控亚洲小车蝗型变中的生理功能奠定基础。【方法】采用高效液相色谱法测定散居型和群居型亚洲小车蝗不同发育阶段(4和5龄若虫及1,4,7,10,13和20日龄成虫)和3龄若虫群居化处理1,3,5和7 d时亚洲小车蝗血淋巴中的保幼激素JHⅢ的滴度变化;用qRT-PCR检测在群居化处理亚洲小车蝗3龄若虫1,3,5和7 d时保幼激素代谢相关的3种基因保幼激素酯酶(juvenile hormone esterase,JHE)基因、保幼激素甲基转移酶(juvenile hormone methyltransferase,JHAMT)基因和保幼激素环氧水解酶(juvenile hormone epoxide hydrolase,JHEH)基因的表达量;利用JHⅢ浸液处理散居型亚洲小车蝗3龄若虫后进行群居化处理,测定亚洲小车蝗型变率。【结果】JHⅢ在亚洲小车蝗血淋巴中的滴度甚微,在4和5龄两型若虫中最大滴度低于50 ng/mL,在两型成虫中最大滴度低于100 ng/mL;JHⅢ滴度随成虫日龄增加而升高,13日龄时达到高峰,然后随日龄增加而缓慢下降,两型雌成虫和雄成虫呈类似的趋势;除1日龄成虫外,其他日龄散居型亚洲小车蝗成虫血淋巴中JHⅢ滴度均显著高于群居型的;与散居型对照比较,群居化处理3龄若虫1-7 d亚洲小车蝗血淋巴中JHⅢ滴度均显著降低。JHE,JHAMT和JHEH基因的表达量在型变过程中发生显著变化,呈现先增后减的趋势。JHⅢ浓度增加可有效抑制散居型亚洲小车蝗向群居型转变。【结论】保幼激素JHⅢ在亚洲小车蝗两型转变中具有重要作用,JHE,JHAMT和JHEH基因在亚洲小车蝗两型转变过程中可发挥不同的功能。【Aim】The purpose of this study is to clarify the changes in the titer of juvenile hormone(JH)JHⅢand the expression levels of its metabolism-related genes in different developmental stages and during phase transition of Oedaleus asiaticus,so as to lay a foundation for elucidating the physiological function of JH in regulating the phase transition of O.asiaticus.【Methods】High performance liquid chromatography was used to determine the titer change of juvenile hormone JHⅢin the haemolymph of O.asiaticus of solitarious and gregarious phases at different developmental stages(the 4th and 5th instar nymphs,and 1-,4-,7-,10-,13-and 20-day-old adults)and at 1,3,5 and 7 d after the 3rd instar nymphs of O.asiaticus were subjected to gregarilization treatment.The expression levels of three kinds of genes related to juvenile hormone metabolism,i.e,juvenile hormone esterase(JHE)gene,juvenile hormone methyltransferase(JHAMT)gene and juvenile hormone epoxide hydrolase(JHEH)gene at 1,3,5 and 7 d after the 3rd instar nymphs of O.asiaticus were subjected to gregarilization treatment were determined by qRT-PCR.The phase transition rate of O.asiaticus was determined after the 3rd instar nymphs were subjected to JHⅢliquid immersion and gregarilization treatment.【Results】The JHⅢtiter in the hemolymph of O.asiaticus was very low,with the maximum titers below 50 ng/mL in the 4th and 5th instar nymphs of the two phases and the maximum titers below 100 ng/mL in adults of the two phases.The JHⅢtiter increased with the increase of the adult day-old age,and reached a peak at the 13-day-old,and then decreased slowly with the increase of the day-old age,showing a similar trend in female and male adults of the both two phases.The JHⅢtiters in the haemolymph of various day-old adults of solitarious phase except for the 1-day-old adults were significantly higher than those of gregarious phase of O.asiaticus.The JHⅢtiters in the haemolymph of O.asiaticus at 1-7 d after the 3rd instar nymphs were subjected to gregarilization

关 键 词：亚洲小车蝗 保幼激素 基因表达 散居型 群居型 型变 

分 类 号：S812.6[农业科学—草业科学]