百合根腐病病原分离与鉴定  

Isolation and Identification of Lily Root Rot Pathogen

作　　者：刘彤珂 宋水林[1,2] 熊桂红 赖家豪 蒋军喜 刘冰[1,2] LIU Tongke;SONG Shuilin;XIONG Guihong;LAI Jiahao;JIANG Junxi;LIU Bing(College of Agriculture,Jiangxi Agricultural University,Nanchang 330045,China;Jiangxi Key Laboratory of Potato and Taro Biology,Nanchang 330045,China)

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]江西省薯芋生物学重点实验室,江西南昌330045

出　　处：《生物灾害科学》2023年第1期1-7,共7页Biological Disaster Science

基　　金：江西省自然科学基金项目(20192BAB214004,20161BAB204184)。

摘　　要：【目的】百合根腐病是严重为害百合鳞茎的一种土传病害,其病原种类多样,已成为遏制百合产业发展的重要因素之一。研究从百合根腐病的病样中分离得到多种分离物,选取其中2种做进一步鉴定。【方法】采用组织分离法对江西省鹰潭市余江区杨溪乡发生的百合根腐病样品进行病原分离,进一步通过形态观察、ITS和tef1序列比对以及致病性测定对病原进行鉴定。【结果】明确引起百合根腐病的病原为藤仓镰刀菌(Fusarium fujikuroi)和茄病镰刀菌百合专化型(Fusarium solani f.sp.lilii Wang)。【结论】研究从江西余江区采集的龙牙百合病鳞茎组织上分离得到两种代表性镰刀菌,分别为藤仓镰刀菌和茄病镰刀菌百合专化型。研究结果为百合根腐病的预防和防治提供了参考依据。[Objective]Lily root rot is a soil-borne disease seriously harming lily bulbs.Its pathogens are diverse and have become one of the important factors to curb the development of lily industry.In this study,a variety of isolates were isolated from lily root rot samples,of which two were selected for further identification.[Method]The pathogens of lily root rot in Yangxi Town,Yujiang County,Jiangxi Province,were isolated by tissue isolation method,and were further identified by morphological observation,ITS and tef1 sequence comparison and pathogenicity test.[Result]The pathogens causing lily root rot were Fusarium fujikuroi and Fusarium solani f.sp.lilii Wang.[Conclusion]In this study,two representative Fusarium species were isolated from the bulbs of Lilium longa collected in Yujiang County,Jiangxi Province.They were F.fujikuroi and F.solani f.sp.lilii Wang.The results provided a reference for the prevention and control of lily root rot.

关 键 词：百合 根腐病 病原鉴定 藤仓镰刀菌 茄病镰刀菌 

分 类 号：S436.631.16[农业科学—农业昆虫与害虫防治]