海洋真菌Pestalotiopsis sp.甾体化合物的分离纯化及抗肿瘤活性研究  

Separation and purification of sterols from marine fungus Pestalotiopsis sp.and study on its anti-tumor activity

作　　者：雷辉 夏童 王佳女 熊霞 刘利 LEI Hui;XIA Tong;WANG Jianü;XIONG Xia;LIU Li(School of Pharmacy,Southwest Medical University,Luzhou 646000,China;the Affiliated Hospital of Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]西南医科大学附属医院,四川泸州646000

出　　处：《中国测试》2023年第3期60-64,共5页China Measurement & Test

基　　金：四川省科技厅项目(2020YJ0191);国家自然科学基金青年项目(82003716)。

摘　　要：为了从海洋真菌次生代谢产物中获得抗肿瘤药物,该文对海洋真菌Pestalotiopsis sp.中的甾体化合物进行分离纯化、结构鉴定和抗肿瘤活性研究。利用硅胶柱色谱,Sephadex LH-20柱色谱等技术,从固体大米培养基发酵产物中分离纯化得到甾体化合物,鉴定为:4,4-dimethyl-5α-ergosta-8,24(28)-dien-3β-ol,该化合物为首次从该属种分离获得,命名为:LH-1。采用MTT方法对该化合物进行体外细胞毒性测试,发现其可以选择性抑制肿瘤细胞的生长(IC 50:8.3µmol/L),而对正常细胞没有明显的杀伤作用。该研究可为微生物来源的抗肿瘤先导化合物的发现提供实验基础。To obtain antitumor drugs from secondary metabolites of marine fungi,the sterol constituents of marine fungus Pestalotiopsis sp.and its antimelanoma activity were studied.One compound was isolated from the extracts of solid rice medium by means of chromatographic techniques,including silica gel column and Sephadex LH-20 column chromatography.The structure was identified as 4,4-dimethyl-5α-ergosta-8,24(28)-dien-3β-ol,which was isolated from the marine fungus Pestalotiopsis sp.for the first time and was named LH-1.The anti-tumor activities of LH-1 was screened by using the MTT.The result showed that compound LH-1 exhibited selective inhibitor activities against anti-tumor cells and not toxicity to normal cells,with IC 50 value of 8.3µmol/L.This study provides an experimental basis for the discovery of antitumor lead compounds from microorganisms.

关 键 词：拟盘多毛孢属 甾体 结构鉴定 抗肿瘤活性 

分 类 号：R932[医药卫生—生药学]