木贼镰刀菌的鉴定及其对桃蚜的致病性测定  

Identification of Fusarium equiseti and Its Pathogenicity to Myzus persicae

作　　者：赵雪怡 柴军发 张暄翊 洪波[1] 贾彦霞[1] ZHAO Xueyi;CHAI Junfa;ZHANG Xuanyi;HONG Bo;JIA Yanxia(School of Agricultural,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《中国生物防治学报》2023年第1期69-76,共8页Chinese Journal of Biological Control

基　　金：宁夏回族自治区农业科技自主创新资金农业高质量发展和生态保护科技创新示范项目(NGSB-2021-10-04)。

摘　　要：从罹病桃蚜虫尸上分离到了一株昆虫病原真菌,命名为JMF-01,本文旨在确定该菌株分类地位并探索其生防潜能。对罹病的桃蚜虫尸进行分离纯化,基于形态学观察和r DNA-ITS、RPB2、IGS序列分析,构建系统发育树对该菌株进行鉴定。采用浸叶浸虫法研究该菌株不同浓度条件下对桃蚜的致病力并进行温室防效测定。结果表明菌株JMF-01在PDA培养基上5 d后菌落直径58~60 mm,分生孢子镰刀形,3~7隔。菌株JMF-01对桃蚜具有较强致病力,处理桃蚜在7 d后累计校正死亡率和LC_(50)达到85%和9.87×10~5cfu/mL;孢子悬浮液最高浓度的温室防效在14 d时高于70%。该菌株的r DNA-ITS序列(MW404610)与木贼镰刀菌Fusarium equiseti(Gen Bank登录号:JF773657)相似度达100%,位于系统发育树的同一分支;RPB2、IGS序列分别与木贼镰刀菌(GenBank登录号:MK077112,KX583611)的相似度也达到99%以上,聚在系统发育树的同一分支。菌株JMF-01经鉴定为桃蚜的病原真菌木贼镰刀菌,具有生防潜力。A strain of entomopathogenic fungus was isolated from the corpse of Myzus persicae and designated as JMF-01.In this study,the author aims to determine the taxonomic status of the strain and explore its biocontrol potential.The strain was isolated and purified from the diseased peach aphid corpse.Based on morphological observation and rDNA-ITS,RPB2 and IGS sequence analysis,the phylogenetic tree was constructed to identify the strain.The pathogenicity of the strain to M.persicae under different spore suspension concentrations was measured by leaf dipping and insect dipping method and the control of the aphids by the strain was determined in greenhouse tests.The results showed that the colony diameter of JMF-01 was 58―60 mm at 5 days on PDA medium,conidia were sickle-shaped and separated by 3―7.Strain JMF-01 showed strong virulence to M.persicae and the cumulative corrected mortality and LC_(50) reached 85%and 9.87×10~5 cfu/mL at 7 days after treatment,respectively.The control efficiency of the aphids by the spore suspension at the highest concentration in greenhouse was higher than 70%at 14 days after treatment.The rDNA-ITS sequence (MW404610) of the strain was 100%consistent with that of Fusarium equiseti (GenBank accession number:JF773657),locating in the same branch of the phylogenetic tree;the similarity of RPB2 and IGS sequences with F.equiseti (GenBank accession number:MK077112,KX583611) also reached more than 99%,clustering in the same branch of the phylogenetic tree.In conclusion,the strain JMF-01 identified as F.equiseti is a virulent pathogen of M.persicae with high biocontrol potential.

关 键 词：桃蚜 木贼镰刀菌 昆虫病原真菌 致病力 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]