黏虫CYP9A134基因的克隆及其解毒功能  

Cloning of CYP9A134 Gene and Its Detoxification Function in Mythimna separata

作　　者：张良[1] 杨洪佳 李泽 张春雨 胡育硕 樊东[1] ZHANG Liang;YANG Hongjia;LI Ze;ZHANG Chunyu;HU Yushuo;FAN Dong(College of Plant Protection,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学植物保护学院,哈尔滨150030

出　　处：《中国生物防治学报》2023年第1期130-140,共11页Chinese Journal of Biological Control

基　　金：现代农业产业技术体系建设专项资金(CARS-04)。

摘　　要：昆虫体内的细胞色素P450酶系统在昆虫解毒过程中发挥着重要作用。本次试验通过转录组测序获得一条新的黏虫P450基因,经国际委员会命名为CYP9A134(登录号为MT990973)。该序列全长为1801 bp,开放序列长度为1596 bp,编码531个氨基酸,分子质量为61.42 kDa,等电点为4.90。在黏虫幼虫阶段用2.5%高效氯氟氰菊酯和20%氯虫苯甲酰胺诱导时该基因表达量会有不同程度上升,最高的可分别达对照组的2.6倍和6.5倍。RNA干扰后,该基因表达量最低下降了70%,以上2种杀虫剂LD30杀虫效果分别提高了13%和25%;在黏虫成虫阶段用20%氯虫苯甲酰胺LD30诱导时,该基因表达量最高可达7.8倍。RNA干扰后,该基因表达量最低下降了61%,LD30剂量的氯虫苯甲酰胺杀虫效果提高26%。结果表明,该基因可能在杀虫剂诱导的解毒代谢过程中发挥重要作用。The cytochrome P450 monooxygenase system in insects plays an important role in detoxification.A novel P450 gene obtained from Mythimna separata by transcriptome sequencing was nominated CYP9A134 by international P450 Nomenclature Committee(Genbank accession number MT990973).The full-length CYP9A134cDNA sequence was 1801 bp with an open reading frame of 1596 bp,which encoded a 531-amino acid polypeptide.The predicted molecular weight of the deduced amino acids was 61.42 kDa and its isoelectronic point was 4.90.The expression level of CYP9A134 was increased in the M.separata larvae upon induction with2.5% beta-cyfluthrinand and 20% chlorantraniliprole,with the highest values reaching 2.6 times and 6.5 times that of the control group,respectively.After RNA interference,CYP9A134 expression level was decreased by 70%,and the mortalities of M.separata was increased by 13% and 25% after treatment with LD_(30) of beta-cyfluthrinand and 20% chlorantraniliprole,respectively.CYP9A134 expression was upregulated 7.8 times when the adults were induced with LD_(30) of 20% chlorantraniliprole.After RNA interference,CYP9A134 expression level was decreased by 61% and the mortality of M separata adults was increased by 26% after treatment with chlorantraniliprole.The results suggest that CYP9A134 may play an important role in insecticide-induced detoxification metabolism.

关 键 词：黏虫 细胞色素P450基因 诱导 RNA干扰 死亡率 

分 类 号：Q96[生物学—昆虫学]