贵州平塘黑糯稻半矮秆突变体bgsd-2苗期与抽穗期转录组比较分析  

Comparison of Transcriptome between Seedling Stage and Heading Stage of Semi-dwarf Mutant bgsd-2 from Guizhou Pingtang Landrace Rice

作　　者：刘慧 薛承进 赵兰馨 赵德刚 黄小贞[1,2] LIU Hui;XUE Chengjin;ZHAO Lanxin;ZHAO Degang;HUANG Xiaozhen(Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China;College of Tea Sciences,Guizhou University,Guiyang 550025,China;Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳550025 [2]贵州大学茶学院,贵阳550025 [3]贵州省农业科学院,贵阳550006

出　　处：《种子》2023年第1期7-12,44,共7页Seed

基　　金：贵州省科学技术基金(黔科合人才[2015]2053);国家自然科学基金(31960615)。

摘　　要：株高是水稻重要的农艺性状,与产量和品质密切相关。突变体bgsd-2在抽穗期与野生型平塘黑糯相比,表现出明显矮化,但是苗期株高没有显著差异。本研究在前期PTWT和bgsd-2抽穗期转录组数据的基础之上,进一步比较分析了PTWT和bgsd-2苗期和抽穗期两个发育阶段差异基因的动态变化。从4个比较组(PTWT-L与PTWT-F、bgsd-2-L与bgsd-2-F、PTWT-L与bgsd-2-L、PTWT-F与bgsd-2-F)中分别鉴定得到3984、3533、2107、1082个差异基因。KEGG分析发现,水稻苗期到抽穗期转变过程中,氨基酸的生物合成、淀粉和蔗糖代谢、碳代谢和核糖体等途径起重要作用。KEGG富集显示,bgsd-2突变位点可能直接影响苯丙烷生物合成和苯丙氨酸代谢两条途径相关基因表达。进一步分析不同比较组中差异转录因子变化的数量和类别,筛选到多个可能在bgsd-2从苗期到抽穗期转变过程中起作用的转录因子。Plant height is an important agronomic trait of rice(Oryza sativa),which determines rice yield and quality.bgsd-2 mutant conpared to Pingtang wild type(PTWT)at the heading stages,it exhibited a dwarf phenotype,but plant height showed no significance at seedling stage.Based on the previous transcriptome data of PTWT and bgsd-2 at heading stage,this study further compared and analyzed the dynamic changes of differential genes between PTWT and bgsd-2 at seedling stage and heading stage.From four comparison groups(PTWT-L and PTWT-F,bgsd-2-L and bgsd-2-F,PTWT-L and bgsd-2-L,PTWT-F and bgsd-2-F),3984,3533,2107 and 1082 differentially expressed genes(DEGs)were identified,respectively.KEGG analysis showed that amino acid biosynthesis,starch and sucrose metabolism,carbon metabolism and ribosome played important roles in the phase change from seedling to heading stage.KEGG enrichment showed that the bgsd-2 mutation might directly affect related gene expressing of two metabolic pathways of phenylalanine metabolism and phenylpropanoid biosynthesis.Further analysis of the number and categories of differential transcription factor changes in different comparison groups,multiple transcription factors that could play a role in the transition of bgsd-2 from seedling to heading stage were identified.

关 键 词：平塘黑糯 半矮秆 bgsd-2 转录组测序 发育时期 

分 类 号：S511[农业科学—作物学] Q812[生物学—生物工程]