籼稻花药培养技术体系优化及高培养力基因型的筛选  

Optimizing of Indica Rice Anther Culture System and Screening of Genotypes with Higher Anther Culture Ability

作　　者：赵沙沙[1] 陈波[1] 房振兵[1] 潘高峰 潘秀才 赵红 田永宏[1] ZHAO Shasha;CHEN Bo;FANG Zhenbing;PAN Gaofeng;PAN Xiucai;ZHAO Hong;TIAN Yonghong(Xiangyang Academy of Agricultural Sciences,Xiangyang Hubei 441057,China)

机构地区：[1]襄阳市农业科学院,湖北襄阳441057

出　　处：《种子》2023年第1期135-140,共6页Seed

基　　金：湖北省农业科技创新中心重大科技研发项目(2020-620-000-002-01);湖北省重点研发计划项目(2020BBB053)。

摘　　要：本研究选用改良M 8、N 6基本培养基和麦芽糖、蔗糖设置4种不同处理水平的诱导培养基进行花药培养。结果表明,对于大部分籼稻材料,诱导愈伤培养基添加N 6培养基和麦芽糖较为理想。以7个籼稻品种(系)为材料进行花药培养,筛选出高花药培养力基因型农香24、襄中稻3号、禾香1号和襄中稻1号,可作为单倍体育种、构建DH群体以及遗传转化的基础材料。In this study,the improved M 8 and N 6 basic media,maltose and sucrose were used as four different treatment levels of induction medium to carry out anther culture.The results showed that callus-inducing medium added with N 6 medium and maltose were ideal for most indica rice.Seven indica rice cultivars(lines)were used for anther culture,and Nongxiang 24,Xiangzhong Dao 3,Hexiang 1 and Xiangzhong Dao 1 with high anther culture ability were screened out,which could be used as basic materials for haploid breeding,DH population construction and genetic transformation.

关 键 词：籼稻 花药培养 基本培养基 碳源 基因型 

分 类 号：S511[农业科学—作物学]