烟农系列小麦高产遗传基础解析  

Genetic basis analysis of high-yielding in Yannong wheat varieties

作　　者：王昊 孙妮娜[1] 王矗 肖露凝 肖蓓 李栋[3] 刘洁[1] 秦冉 吴永振 孙晗 赵春华 李林志[1] 崔法 刘伟[1] WANG Hao;SUN Ni-Na;WANG Chu;XIAO Lu-Ning;XIAO Bei;LI Dong;LIU Jie;QIN Ran;WU Yong-Zhen;SUN Han;ZHAO Chun-Hua;LI Lin-Zhi;CUI Fa;LIU Wei(Yantai Academy of Agricultural Sciences,Yantai 265500,Shandong,China;College of Life Sciences,Yantai University,Yantai 264005,Shandong,China;Shandong Provincial Seed Management Station,Jinan 250131,Shandong,China;College of Agriculture,Ludong University/Key Laboratory of Crop High Yield and Stress Resistance Molecular Modules in Shandong Higher Education Institutions,Yantai 264025,Shandong,China)

机构地区：[1]山东省烟台市农业科学研究院,山东烟台265500 [2]烟台大学生命科学学院,山东烟台264005 [3]山东省种子管理总站,山东济南250131 [4]鲁东大学农学院/山东省高等学校作物高产抗逆分子模块重点实验室,山东烟台264025

出　　处：《作物学报》2023年第6期1584-1600,共17页Acta Agronomica Sinica

基　　金：山东省农业良种工程项目(2019LZGC001);山东省高等学校青创科技支持计划项目(2019KJF002);烟台市科技计划项目(2022XCZX092);财政部和农业农村部国家现代农业产业技术体系建设专项(小麦,CARS-3-2-23);烟台市2022年种子工程攻坚项目--小麦育种攻关联合团队创建项目资助

摘　　要：烟农系列小麦品种具有高产、抗病、广适性等特点,近几年审定的高产多抗品种烟农1212,曾多次打破全国冬小麦单产纪录,鲁麦14已衍生出至少214份小麦新品种,成为重要的骨干亲本。本研究旨在解析烟农系列小麦高产遗传基础,明确其高产广适关键染色体区段,为小麦新品种遗传改良提供理论参考。利用小麦55K SNP芯片对38份烟农系列小麦品种、其部分衍生后代品种和244份育成品种(系)组成的自然群体进行基因型扫描,并进行了多环境表型鉴定。基因型分析结果表明,自主选育的17份烟农品种之间的遗传相似系数在0.80~0.99之间,获得了975个高频率共同选择区段,区段长度变幅为1.00~75.18 Mb,其中在2D、4D、6D、7B染色体上存在较多的高频率共同选择区段,其总长度占对应染色体的40%以上。骨干亲本鲁麦14对其23个衍生后代的平均遗传贡献率为71.45%,在3个(A、B、D)亚基因组的贡献率分别为69.63%、66.04%和79.82%;共检测到430个在鲁麦14衍生后代中高频率选择遗传区段,265个区段(61.6%)与烟农系列高频率共同选择区段重叠。基于自然群体表型鉴定及遗传解析结果显示,骨干亲本鲁麦14和最新选育的高产广适主推品种烟农1212均富集了千粒重和单株产量优异等位基因;在鲁麦14高频率选择区段上富集了92.3%和84.4%的来自鲁麦14的增加千粒重和单株产量显著关联SNP位点,主要分布在2A、2B、2D、4A、5B、6A、7A染色体上。烟农系列小麦高频率共同选择基因组区段已富集了丰富的高产基因位点,是其高产稳产的遗传基础所在。The Yannong series wheat has the characteristics of high yield potential,disease resistance,and wide adaptability.Yannong 1212,a high-yield and multi-resistant variety approved in recent years,has repeatedly broken the national winter wheat yield record for many times.To date,the numerous varieties derived from Yannong series have been released.Lumai 14 has derived from at least 214 wheat authorized varieties and become an important backbone parent by 2020.This objective of the study is to characterize genetic basis of high yielding potential in Yannong series,identify the key chromosomal segments of high yield and wide adaptability,and provide theoretical reference for the genetic improvement of new wheat varieties.The genotype of 38 Yannong series wheat varieties,some of their derived varieties,and 244 authorized varieties(advanced lines)were scanned by wheat 55K SNP array,and the environmental phenotypes were identified.Phenotype evaluation of yield-related traits of the natural mapping population was conducted in multiple environments.The genetic effects of the common high-frequency co-selected genetic segment(HFCS)of Yannong series varieties were characterized in detail based on the genotypes of wheat 55K SNP array.In addition,the HFCS from Lumai 14 to its derived varieties were also specified.The genetic basis of high yield potential in Yannong 1212 and Lumai 14 were revealed at the whole genome-wide level.The genetic similarity coefficients among the 17 Yannong series authorized varieties ranged from 0.80 to 0.99.Based on genotyping data from the 55K SNP array in the 17 Yannong series authorized varieties,975 HFCS were obtained,and their segments lengths ranged from 1.00 Mb to 75.18 Mb.Most HFCS were distributed on chromosomes 2D,4D,6D,and 7B,with the total length accounting for more than 40%of the corresponding chromosomes,respectively.The genetic contribution rate of Lumai 14 to its 23 derived varieties was approximately 71.45%on average at the genome level,with A,B,and D of 69.63%,66.04%,and 79.82%,resp

关 键 词：小麦 遗传贡献 55K SNP芯片 遗传区段 骨干亲本 

分 类 号：S512.1[农业科学—作物学]