月见草茎提取物对2型糖尿病小鼠的治疗作用  

The therapeutic Effect of Oenothera biennis Stem Extraction on Type 2 Diabetic Mice

作　　者：伍俊衡 彭志豪 祝浩翔 廖子劼 唐亮[1] WU Junheng;PENG Zhihao;ZHU Haoxiang;LIAO Zijie;TANG Liang(Institute of Basic Medical College,Changsha Medical College,Changsha 410219,China)

机构地区：[1]长沙医学院基础医学院,湖南长沙410219

出　　处：《特产研究》2023年第2期90-94,109,共6页Special Wild Economic Animal and Plant Research

基　　金：湖南省省级大学生创新项目(湘教通〔2021〕197号-3876,S202110823032)。

摘　　要：明确月见草茎提取物对2型糖尿病小鼠干预效果。36只C57/BL6J小鼠随机分为6组:正常对照组,2型糖尿病模型组、二甲双胍阳性对照组以及月见草茎提取物高、中、低浓度组。除正常对照组外,其他5组采用高糖饲料喂养,结合STZ辅助诱导建立2型糖尿病小鼠模型。月见草茎提取物高、中、低浓度组分别采用1.0 g/mL、0.5 g/mL和0.25 g/mL月见草茎提取物溶液灌胃(0.1 mL/10g)。正常对照组和2型糖尿病模型组灌胃等体积生理盐水。每天给药1次,持续60d。每间隔10d检测小鼠体重、空腹血糖及糖耐量。60d后,摘眼球取血,紫外分光光度法检测血清MDA、T-SOD和GSH-PX水平,HE染色检测胰岛数量及形态变化。与模型组相比,月见草茎提取物有明显的降血糖改善糖耐量、抑制氧化应激、稳定体重水平和保护胰岛细胞的作用(P<0.05),并呈现剂量和效果的依赖关系。给药60d后,糖尿病的症状改善效果明显,与2型糖尿病模型组相比,具有统计学差异(P<0.05)。月见草茎提取物对2型糖尿病模型小鼠有治疗作用,并存在量效关系。we aimed to study the intervention effect of Oenothera biennis L.stem extraction on type 2 diabetic(T2D)mice.36 C57/BL6J mice were randomly divided into 6 groups:normal control group,T2D model group,metformin-positive control group,high-concentration,medium-concentration,and low-concentration of O.biennis stem extraction groups.Except to the normal control group,the other 5 groups were fed with high sugar diet.And the T2D mice models were established by STZ-assisted induction.The mice in high,medium and low concentration groups were separately given 1.0 g/mL,0.5 g/mL and 0.25 g/mL O.biennis stem extraction by intragastric administration(0.1 mL/10 g).Normal control group,T2D model group intragastric equal volume of normal saline.The drug was administered once a day for 60 days.The body weight,fasting blood glucose and glucose tolerance ofmice were measured in every 10 days.After 60 days,the eyeballs were taken for blood.The levels of MDA,T-SOD and GSH-PX in serum were detected by UV spectrophotometry.HE staining was used to detect the number and morphology of islets.Compared with the model group,stem extract of O.biennis showed obvious effects of reducing blood glucose,improving the glucose tolerance,inhibiting oxidative stress,stabilizing body weight and protecting islet cells(P<0.05),and showed a dose-dependent relationship.After administrated for 60 days,the improvement effect of diabetes symptoms was significant,and the difference was statistically significant compared with T2D model group(P<0.05).O.biennis stem extraction has a therapeutic effect on T2D model mice,and there is a dose-effect relationship.

关 键 词：月见草 2型糖尿病 氧化应激 胰岛 

分 类 号：R285.5[医药卫生—中药学]