利用微型离心管提取分析天然橡胶主要成分聚异戊二烯方法的建立  

Establishment of A Microtube Extraction Method for Natural Rubber Basic Ingredient Polyisoprene Analysis

作　　者：张君 雍婧姣 路国栋 安迎瑞[1,2,3] 张虹 郎思睿 陈任[1,2,3] ZHANG Jun;YONG Jing-jiao;LU Guo-dong;AN Ying-rui;ZHANG Hong;LANG Si-rui;CHEN Ren(Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University,Yinchuan 750021,Ningxia,China;School of Life Science,Ningxia University,Yinchuan 750021,Ningxia,China;Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Ningxia University,Yinchuan 750021,Ningxia,China)

机构地区：[1]宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川750021 [2]宁夏大学生命科学学院,宁夏银川750021 [3]宁夏大学宁夏优势特色作物现代分子育种重点实验室,宁夏银川750021

出　　处：《西北林学院学报》2023年第2期223-228,234,共7页Journal of Northwest Forestry University

基　　金：国家自然科学基金目(31960065);宁夏回族自治区重点研发计划(重大)重点项目(2019BFG02011)。

摘　　要：为建立一种快速、准确的从少量样品中提取、分析天然橡胶主要成分聚异戊二烯含量的方法,在已采用索氏提取器提取的基础上,进一步探讨采用2 mL微型离心管的方法提取分析材料(0.5 g以下)中的聚异戊二烯含量。从分别利用微型离心管与索氏提取器提取4种样品分析材料,即顺式聚异戊二烯标准品、多汁乳菇子实体、杠柳枝条、杜仲果翅的提取得率和分子量分布看,2种提取方法的差别不大,再通过每次提取时利用顺式聚异戊二烯标准品建立标准曲线校正,采用2 mL微型离心管微量提取分析测定植物植株、组织材料或真菌子实体、菌丝体中的顺式或反式-1、4-聚异戊二烯橡胶含量的方法可行、结果可信。In order to establish a rapid,accurate analysis method for the content test of polyisoprene,the basic ingredient in natural rubber that extracted from a small quantity of sample,based on the previous method by Soxhlet extractor,the extraction and analysis of polyisoprene from sample(less than 0.5 g)in 2 mL microtube was further discussed.Four kinds of samples,cis-polyisoprene standard,Lactarius volemus fruiting body,Periploca sepium branch and Eucommia ulmoides winged perianth,were extracted by microtube and Soxhlet extractor respectively.No significant differences were found in extraction rate and molecular weight distribution between the two methods.In addition,the content of polyisoprene could be corrected by the standard curve established by cis-polyisoprene standards during each extraction.The 2 mL microtube extraction method is feasible and reliable to analyze the content of cis-or trans-1,4-polyisoprene in plant plantlet,tissue materials or fungi fruiting bodies,mycelia.

关 键 词：天然橡胶 聚异戊二烯 微型离心管提取 索氏提取器提取 提取得率 分子量分布 

分 类 号：S718.43[农业科学—林学]