呼吸道合胞病毒感染Hep-2及Vero细胞的病变特点及易感性分析  

Pathological characteristics and susceptibility analysis of Hep-2 and Vero cells infected with respiratory syncytial virus

作　　者：叶芝旭 张梅[2] 金鑫[3] 阳小凤 崔玉霞[1] 余福勋 YE Zhixu;ZHANG Mei;JIN Xin;YANG Xiaofeng;CUI Yuxia;YU Fuxun(Department of Pediatrics,Guizhou Provincial People's Hospital,Guizhou Province,Guiyang 550002,China;Department of Biomedical Sciences,Guizhou University,Guizhou Province,Guiyang 550000,China;Department of Ophthalmology,Guizhou Provincial People's Hospital,Guizhou Province,Guiyang 550002,China;Department of Pediatrics,the First Clinical College,Zunyi Medical University,Guizhou Province,Zunyi 563000,China;Central Laboratory,Guizhou Provincial People's Hospital,Guizhou Province,Guiyang 550002,China)

机构地区：[1]贵州省人民医院儿科,贵州贵阳550002 [2]贵州大学生物医学系,贵州贵阳550000 [3]贵州省人民医院眼科,贵州贵阳550002 [4]遵义医科大学第一临床学院儿科,贵州遵义563000 [5]贵州省人民医院中心实验室,贵州贵阳550002

出　　处：《中国当代医药》2023年第9期7-10,17,F0003,共6页China Modern Medicine

基　　金：国家自然科学基金委员会资助项目(81860003、81960001);贵州省科技计划项目(黔科技支撑[2018]2785);贵州省卫生计生委科学技术基金项目(gzwjw2018-1-049);贵州省高层次创新型人才“千层次”(GZSYQCC【2023】011)。

摘　　要：目的比较Hep-2细胞与Vero细胞感染呼吸道合胞病毒(RSV)的病变特点、易感性,及RSV在两种细胞上产生的子代病毒的感染性。方法实验分为Hep-2空白对照组、Hep-2实验组、Vero空白对照组及Vero实验组。空白对照组未感染病毒,实验组为RSV以MOI=1感染细胞。RSV感染两组细胞后,显微镜观察细胞病变效应(CPE),免疫荧光检测RSV N蛋白表达及空斑实验测定病毒滴度,并进一步予Hep-2实验组及Vero实验组产生的子代病毒感染A549细胞,空斑实验比较子代病毒的感染性。结果Hep-2实验组感染后48 h,Vero实验组感染后84 h时均可形成明显的合胞病变,两个实验组的细胞病变特点存在明显差异,免疫荧光检测RSV N蛋白表达特点与细胞病变特点一致,两个实验组均能产生的较高的病毒滴度。但当Hep-2实验组及Vero实验组产生的子代病毒感染A549细胞,发现感染后36、48 h,两组细胞的子代病毒滴度比较,差异无统计学意义(P>0.05),感染后60 h,Hep-2实验组细胞的子代病毒滴度高于Vero实验组,差异有统计学意义(P<0.05)。结论RSV感染Hep-2及Vero细胞后均可形成明显的合胞病变,均能达到较高病毒滴度,但RSV在Hep-2细胞上产生的子代病毒感染性明显强于Vero细胞产生的子代病毒。Objective To compare the pathological characteristics and susceptibility of HEP-2 cells and Vero cells infected with respiratory syncytial virus(RSV),and the infectivity of the progeny virus produced by RSV on the two kinds of cells.Methods The experiment was divided into Hep-2 blank control group,Hep-2 experimental group,Vero blank control group and Vero experimental group.The blank control group was not infected with virus,and the experimental group was infected with RSV at MOI=1.The cytopathic effect(CPE)of cells was observed by microscope,the expression of RSV N protein was detected by immunofluorescence,and the virus titer was determined by plaque assay.A549 cells were further infected with the progeny viruses produced by Hep-2 and Vero.The infectivity of progeny viruses was compared by plaque assay.Results At 48 h after infection in Hep-2 experimental group and 84 h in Vero experimental group,obvious CPE were formed in both groups.The characteristics of CPE formed by RSV on Hep-2 and Vero cells were obviously different.The expression of RSV N protein detected by immunofluorescence was consistent with the characteristics of CPE.Both experimental groups were able to produce high levels of virus titers.However,when A549 cells were infected with the progeny virus of produced by Hep-2 and Vero cells,it was found that there was no significant difference in the titer between the two groups at 36 and 48 h after infection(P>0.05),and the titer of of Hep-2 experimental group was higher than that of Vero experimental group at 60 h after infection,with statistically significant difference(P<0.05).Conclusion Both Hep-2 and Vero cells infected with RSV could form obvious CPE,and reach a high virus titer.However,the infectivity of the progeny virus produced by RSV on Hep-2 cells was significantly stronger than that produced on Vero cells.

关 键 词：呼吸道合胞病毒 细胞病变效应 易感性 子代病毒 病毒滴度 

分 类 号：R373[医药卫生—病原生物学]