内蒙古赤峰地区蒙古族35个Y-STR和3个Y-InDel的遗传多态性  

Genetic Polymorphisms of 35 Y-STR Loci and 3 Y-InDel Markers among Mongolian Males of Chifeng City,Inner Mongolia

作　　者：张润 高珊 王珍 沈淼淼 王旭 杜猛 ZHANG Run;GAO Shan;WANG Zhen;SHEN Miaomiao;WANG Xu;DU Meng(Department of Forensic Medicine,Criminal Investigation Police University of China,Shenyang 110854,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)

机构地区：[1]中国刑事警察学院法医系,沈阳110854 [2]公安部鉴定中心,北京100038

出　　处：《刑事技术》2023年第2期172-175,共4页Forensic Science and Technology

基　　金：公安部科技强警基础工作专项(2020GABJC12)

摘　　要：调查35个Y-STR基因座和3个Y-InDel标记在内蒙古赤峰地区蒙古族的分布情况并计算与其他蒙古族男性群体的遗传距离。应用YfilerTM Platinum复合扩增试剂盒检测1318名赤峰地区蒙古族无亲缘关系的男性个体在35个Y-STR基因座和3个Y-InDel标记的基因分型。32个Y-STR单拷贝基因座分别检测到4~16种“等位”基因;3个Y-STR多拷贝基因座(DYS527、DYF387S1、DYS385)分别检测到60、59、79种“等位”基因组合。单倍型多样性为0.999974。经与YHRD数据库中其他蒙古族男性群体共有的25个Y-STR基因座统计数据比较,发现赤峰地区蒙古族男性与呼和浩特蒙古族遗传距离最近(Rst=0.0516),与呼伦贝尔蒙古族遗传距离最远(Rst=0.1091)。所检测35个Y-STR基因座和3个Y-InDel标记在内蒙古赤峰地区蒙古族男性群体中具有丰富的遗传多态性。The 35 Y-STR loci and 3 Y-InDel markers were here surveyed about their distribution and polymorphisms among Mongolian males from Inner Mongolia’s Chifeng city so as to compare the relevant genetic distances against other Mongolian male groups.With utilizaition of Y filerTM Platinum kit,the 35 Y-STR loci and 3 Y-InDel markers were amplified and tested from 1318 healthy Mongolian unrelated males within Chifeng area,having their gene frequencies calculated along with the gene/haplotype diversity and genetic distances.Statistical analysis showed that there were 4 to 16 different genotypes observed within 32 single-copy Y-STR loci and 60,59,79 alleles from the 3 respective multi-copy Y-STR loci of DYS527,DYF387S1 and DYS385,with the resultant haplotype diversity(HD)value being 0.999974 among the 35 Y-STR loci.The genetic distance got its result from calculating against the other 4 Mongolian male groups which were available from YHRD database relating to 25 Y-STR loci co-shared with the here-investigated male people,consequently showing that the Mongolian males from Chifeng area were closest to the Hohhot-residing Mongolian males(Rst=0.0516)and farthest to the Hulun Buir-dwelling Mongolian males(Rst=0.1091).The here-surveyed 35 Y-STR loci and 3 Y-InDel markers revealed abundant genetic polymorphisms among the Mongolian males living in Chifeng area of Inner Mongolia.

关 键 词：Y-STR基因座 Y-InDel标记 遗传距离 基因多样性 蒙古族 

分 类 号：DF795.2[医药卫生—法医学]