间歇温度处理对育成蛋鸡采食及肠道发育的影响  

Effects of Intermittent Different Temperature on Feeding and Intestinal Development of Growing Laying Hens

作　　者：刘增民 潘亚丽 林海[1] 焦洪超 赵景鹏[1] 王晓鹃 LIU ZengMin;PAN YaLi;LIN Hai;JIAO HongChao;ZHAO JingPeng;WANG XiaoJuan(College of Animal Science and Technology,Shandong Agricultural University/Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,Tai’an 271018,Shandong)

机构地区：[1]山东农业大学动物科技学院/山东省动物生物工程与疾病防治重点实验室,山东泰安271018

出　　处：《中国农业科学》2023年第7期1391-1400,共10页Scientia Agricultura Sinica

基　　金：国家重点研发计划(2018YFE0128200);山东省重点研发计划(2019JZZY020602);国家现代农业产业技术体系建设专项资金(CARS-40-K09);山东省“双一流”奖补资金;泰山学者项目(201511023)。

摘　　要：【目的】研究环境温热对家禽采食的影响,补充蛋鸡饲养过程中的参数缺失,为蛋鸡的正确饲养提供科学依据。【方法】选取11周龄的伊莎褐蛋鸡360只,分为5个处理组,每组6个重复,每个重复12只鸡。正式试验前将试验蛋鸡分别转入5间智能环控鸡舍预饲1周。采用标准笼养,每笼3只鸡。鸡舍内相对湿度保持在60%,育成期每天光照8 h(9:00—17:00光照)。分为对照组和4个处理组,对照组保持基础温度22℃不变;4个处理组采用每日间歇温度处理,即每天10:00—18:00期间分别进行24℃、26℃、28℃、30℃的温度处理,其余时间恢复到基础温度22℃(升温与降温时间均在1 h以内)。试验期8周。试验蛋鸡自由采食和饮水,每周统计采食量,每两周采集一次样品,每组随机挑选12只鸡,称重后断颈处死,称量腺胃重量,以及十二指肠、空肠和回肠的重量与长度。采集下丘脑、腺胃和十二指肠样品于液氮中速冻,-80℃保存。试验结束前连续3 d分别在热处理期和非热处理期统计采食量。【结果】在试验的前4周,与对照组T22相比,T30组的采食量显著降低(P<0.05);在试验后4周时,T24组的采食量显著高于T28组和T30组(P<0.05)。在热处理期,T30组的采食量显著低于T22和T24;在非热处理期,T30组的采食量显著低于T22、T24和T26组(P<0.05);各组热处理期的采食量均显著低于非热处理期(P<0.05),并且保持T24组采食量最高,T30组采食量最低。通过统计器官指数,发现在16周时,与T22组相比,各处理组的腺胃指数极显著升高(P<0.01);18周时,与T22组相比,T30组的空肠指数显著降低,且低于其他处理组(P<0.05);20周时,与T22组相比,T24组的腺胃指数、空肠指数和回肠指数显著升高(P<0.05),且空肠指数显著高于T30组(P<0.05)。检测食欲基因的表达,发现在14周时,与T22组相比,各处理组下丘脑NPY(Neuropeptide Y)的表达量显著升高(P<0.05),T30组下丘脑AgRP(Agouti-rela【Objective】The objective of this study was to study the effects of ambient temperature on feeding and intestinal development of poultry,and to supplement the absent temperature parameters for laying hens rearing,so as to provide a certain scientific basis for the correct feeding of laying hens.【Method】A total of 360 Issa brown laying hens aged 11 weeks were selected and divided into 5 treatment groups with 6 replicates per group and 12 hens per replicate.The experimental laying hens were transferred to 5 intelligent environmental control chicken houses for 1 week of pre-trial and 8 weeks of formal experiment,with 3 chickens per cage.The relative humidity in the chicken house was kept at 60%,and the light was kept for 8 h(9:00-17:00)every day during the prelay period.The temperature of the control group was kept unchanged at 22℃,and the four treatment groups were carried out in a manner of daily intermittent,including 24℃,26℃,28℃,and 30℃at 10:00-18:00 every day,respectively,and changed to the base temperature 22℃for the rest of the time,the heating and cooling time were within 1 h.The experiment lasted for 8 weeks.The experimental laying hens were free to eat and drink,the feed intake was counted weekly,and the samples were collected once every two weeks.Twelve hens in each group were randomly selected and weighed,and then killed by neck cutting.The weight of glandular stomach,the weight and length of duodenum,jejunum and ileum were weighed.Hypothalamus,glandular stomach and duodenum samples were frozen in liquid nitrogen and stored at-80℃.Feed intake was calculated in heat treatment period and non-heat treatment period for 3 days before the end of experiment.【Result】Compared with T22 group,the feed intake in T30 group was significantly decreased at 13-16 week(P<0.05);the feed intake in T24 group was significantly higher than that in T28 and T30 groups at 17-20 week(P<0.05).During the heat treatment period,the feed intake of T30 group was significantly lower than that in T22 and T24 group

关 键 词：蛋鸡 采食 温热 育成期 肠道 下丘脑 

分 类 号：S831.4[农业科学—畜牧学]