固本化湿降脂汤调控STING-IRF3通路对脾虚湿阻型单纯性肥胖大鼠炎症细胞因子的影响  

Effect of Guben Huashi Jiangzhi Decoction on inflammatory cytokines in simple obesity rats with spleen deficiency and dampness resistance by regulating STING-IRF3 pathway

作　　者：张琦[1] 朱绚绚 孙勇兵[1] 王美君 谢情江 熊佳丽 周丽[1] 陈浩[1,2] ZHANG Qi;ZHU Xuanxuan;SUN Yongbing;WANG Meijun;XIE Qingjiang;XIONG Jiali;ZHOU Li;CHEN Hao(Jiangxi University of Chinese Medicine,Nanchang 330004,China;The Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang 330006,China)

机构地区：[1]江西中医药大学,江西南昌330004 [2]江西中医药大学附属医院,江西南昌330006

出　　处：《广东药科大学学报》2023年第2期86-93,共8页Journal of Guangdong Pharmaceutical University

基　　金：国家自然科学基金(81860630);江西省卫生健康委员会科技计划项目(202131055);江西省中医药管理局科技计划项目(2020A0299);江西省教育厅科学技术研究项目(GJJ201254)。

摘　　要：目的 探讨固本化湿降脂汤通过STING-IRF3信号通路对脾虚湿阻型单纯性肥胖大鼠炎症细胞因子的影响。方法 建立脾虚湿阻型单纯性肥胖模型后,将大鼠随机分成模型组、奥利司他阳性对照组(37.5 mg/kg)、固本化湿降脂汤高剂量组(34.4 g/kg)、中剂量组(17.2 g/kg)、低剂量组(8.6 g/kg)及正常组,每组8只,灌胃给药1次/d,连续4周。比较各组大鼠体质量、Lee’s系数、远红外热成像及脑部核磁共振成像(MRI),观察固本化湿降脂汤的减肥效果;比较各组大鼠炎症细胞因子如肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、C反应蛋白(CRP)表达变化;采用免疫组织化学法检测脂肪中STING的表达;采用RT-PCR法检测肝脏STING的mRNA表达,使用蛋白印迹法在蛋白水平测量IRF3的变化,观察其对大鼠STING-IRF3信号通路的影响。结果 固本化湿降脂汤干预脾虚湿阻型单纯性肥胖大鼠后体质量下降明显(P<0.05),脑部、腹部异常区域恢复,可有效降低大鼠TNF-α、IL-6、IL-1β、CRP等相关炎症细胞因子的表达(P <0.01),用药前后STING-IRF3通路变化差异明显(P <0.01)。结论 固本化湿降脂汤通过调控STING-IRF3通路,降低脾虚湿阻型单纯性肥胖大鼠体质量并调节相关炎症细胞因子的表达。Objective To investigate the effect of Guben Huashi Jiangzhi Decoction on inflammatory cytokines in simple obesity rats with spleen deficiency and dampness resistance through STING-IRF3 signaling.Methods After establishing the simple obesity rat model of spleen deficiency and dampness stagnation type,the rats were randomly divided into the model group,orlistat positive control group(37.5 mg/kg),Guben Huashi Jiangzhi Decoction high-dose group(34.4 g/kg),medium dose group(17.2 g/kg) and low-dose group(8.6 g/kg),and the normal group,with 8 rats in each group.The drug was given by gavage once a day for 4 weeks.The body mass,Lee's index,far-infrared thermography and magnetic resonance imaging(MRI) of the brain of rats were measured in each group.The expression of inflammatory cytokines tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),IL-1β,C-reactive protein(CRP) were measured in each group.The expression of STING in fat was detected by immunohistochemistry.The expression of STING mRNA in liver was measured by RT-PCR.The changes of IRF3protein were measured by western blotting to observe its effect on STING-IRF3 pathway.Results Guben Huashi Jiangzhi Decoction significantly decreased the body mass of simple obese rats with spleen deficiency and dampness resistance(P<0.05),restored abnormal areas of the brain and abdomen,and reduced the expression of related inflammatory cytokines TNF-α,IL-6,IL-1β and CRP in rats(P<0.01).The change of STING-IRF3pathway was significantly different before and after Guben Huashi Jiangzhi Decoction treatment(P<0.01).Conclusion Guben Huashi Jiangzhi Decoction can reduce the body mass and inflammatory cytokine level in simple obesity rats with spleen deficiency and dampness obstruction by regulating the STING-IRF3 pathway.

关 键 词：固本化湿降脂汤 脾虚湿阻型单纯性肥胖 炎症细胞因子 STING-IRF3通路 

分 类 号：R285.5[医药卫生—中药学]