冬瓜首雌花节位遗传分析与基因定位  

Genetic analysis and gene mapping for position of the first female flower node(FFFN)in wax gourd

作　　者：王敏 刘微 谢大森[1,2] 江彪 闫晋强[1,2] 彭庆务 何晓明 杨松光[1,2] 刘文睿[1,2] WANG Min;LIU Wei;XIE Dasen;JIANG Biao;YAN Jinqiang;PENG Qingwu;HE Xiaoming;YANG Songguang;LIU Wenrui(Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640,China;Guangdong Key Labrratrry for New Technology Research of Vegetables,Guangzhou,Guangdong 510640,China)

机构地区：[1]广东省农业科学院蔬菜研究所,广东广州510640 [2]广东省蔬菜新技术研究重点实验室,广东广州510640

出　　处：《西北农林科技大学学报（自然科学版）》2023年第4期94-101,109,共9页Journal of Northwest A&F University(Natural Science Edition)

基　　金：广东省重点领域研发计划项目(2020B020220003);广东省科技计划项目(2019A050520002);广东省农业科学院中青年学科带头人项目(R2020PY-JG003);广东省农业科学院农业优势产业学科团队建设项目(202103TD);国家自然科学基金项目(32002038)。

摘　　要：【目的】对冬瓜首雌花节位基因(FFFN)进行遗传分析和定位,为FFFN基因的克隆奠定基础。【方法】以首雌花节位差异显著的冬瓜高代自交系材料B214(P_(1))和B227(P_(2))及以P_(1)为母本和P2为父本构建的F1(P_(1)×P_(2))、F_(2)、B_(1)(F_(1)×P_(1))和B_(2)(F_(1)×P_(2))遗传群体为材料,采用6世代混合模型分析方法对FFFN进行遗传分析,并利用已构建的高密度SNP遗传图谱进行数量性状位点(QTL)分析。【结果】冬瓜FFFN遗传符合E模型,主要受2对主基因的加性效应及2对主基因的加性互作效应控制,同时受环境影响较大;结合已构建的高密度分子遗传图谱,仅检测到1个与FFFN相关的QTL位点(FFFN2.1),其对数优势比阈值(LOD)为11.7,贡献率为32.1%,位于2号染色体上的Marker 61668-Marker 39179,物理距离为7.64 Mb。通过标记加密将FFFN2.1定位在InDel2和SSR91之间的1.38 Mb范围。候选区间内有28个候选基因(Bhi02M001022~Bhi02M001049),其中11个基因没有功能注释,其他17个注释的功能基因包括生长素反应蛋白(ARF)、逆转录酶、丝氨酸/苏氨酸蛋白磷酸酶、三角状五肽重复蛋白等。【结论】冬瓜FFFN是多基因控制的数量性状,主效位点FFFN2.1定位在InDel2和SSR91之间,结合功能注释推测Bhi02M001033为候选基因。【Objective】The genetic analysis and gene mapping of the position of the first female flower node(FFFN)of wax gourd were conducted to provide basis for its cloning.【Method】B214 and B227 with significantly different FFFN were selected as parents P_(1)(B214)and P_(2)(B227)for the construction of F_(1)(P_(1)×P_(2)),F_(2),B_(1)(F_(1)×P_(1)),and B_(2)(F_(1)×P_(2))generations.The FFFN genetic analysis was carried out using joint segregation analysis and a high density SNP genetic map was used to detect QTLs.【Result】The FFFN inheritance was fitted into E model,mostly controlled by additive effects and additive×additive interaction of the two major genes and affected by environment.Combined with the constructed high density molecular genetic map,only one QTL locus(FFFN2.1)associated with FFFN was detected with logarithmic odds ratio threshold(LOD)value of 11.7 and contribution rate of 32.1%between Marker 61668-Marker 39179 on Chr.2 with physical distance of 7.64 Mb.By fine mapping,the FFFN2.1 was located in the 1.38 Mb region between InDel2 and SSR91.There were 28 candidate genes(Bhi02M001022-Bhi02M001049),among which 11 had unknown functions and the other 17 encoded functions related to auxin-responsive protein,reverse transcriptase,serine/threonine-protein phosphatase and pentatricopeptide repeat-containing protein.【Conclusion】Wax gourd FFFN was controlled by multiple genes.The main QTL locus FFFN2.1 was mapped between InDel2 and SSR91.Combing gene functional annotation,it was suggested that Bhi02M001033 was the possible candidate gene related to FFFN.

关 键 词：冬瓜 首雌花节位 遗传分析 基因定位 候选基因 

分 类 号：S462.3[农业科学—植物保护]