马钱苷对严重烧伤大鼠肠道屏障的保护作用机制研究  

Protective effect and mechanism of loganin on intestinal barrier in severely burned rats

作　　者：温海玲[1] 杨景哲[1] 孟祥熙 张祥云 WEN Hai-ling;YANG Jing-zhe;MENG Xiang-xi;ZHANG Xiang-yun(Department of Burn and Plastic Surgery,South District Hospital,Affiliated Hospital of Chengde Medical University;Department of Outpatient,Hebei Military Region Chengde Retired Cadres Rest Center,Chengde 067000,Hebei,China)

机构地区：[1]承德医学院附属医院南院区烧伤整形科 [2]河北省军区承德离职干部休养所门诊部,河北承德067000

出　　处：《川北医学院学报》2023年第3期299-302,共4页Journal of North Sichuan Medical College

基　　金：河北省承德市科学技术研究与发展计划项目(202006A165)。

摘　　要：目的:探讨马钱苷对严重烧伤大鼠肠道屏障的保护作用及分子机制。方法:将30只雄性SD大鼠随机分为对照组、烧伤组与马钱苷组,每组各10只。将烧伤组及马钱苷组大鼠背部造成30%TBSA III度烧伤,将对照组大鼠模拟致假烧伤。马钱苷组大鼠予以5 mg马钱苷灌胃,对照组、烧伤组均予以等量生理盐水灌胃,1次/d,持续7 d。给药结束后,采集大鼠门静脉血,采用异硫氰酸荧光素(FITC)-葡萄糖荧光探诊示踪法检测肠道通透性;采用蛋白印迹法检测大鼠肠黏膜组织白细胞介素-1β(IL-1β)、IL-6蛋白表达水平,并检测大鼠回肠组织沉默调节蛋白1(SIRT1)、核转录因子-κB(NF-κB)p65蛋白表达水平。结果:与对照组比较,烧伤组、马钱苷组肠道通透性均增高(P<0.05),但马钱苷组低于烧伤组(P<0.05);烧伤组、马钱苷组肠黏膜IL-1β、IL-6水平增高(P<0.05),但马钱苷组肠低于烧伤组(P<0.05)。与对照组比较,烧伤组和马钱苷组回肠组织SIRT1表达水平下降(P<0.05),NF-κB p65表达水平增高(P<0.05),但与烧伤组比较,马钱苷组回肠组织SIRT1表达水平增高(P<0.05),NF-κB p65表达水平降低(P<0.05)。结论:马钱苷对严重烧伤大鼠肠道屏障具有保护作用,其机制可能与介导SIRT1/NF-κB信号通路、抑制炎症反应有关。Objective:To investigate the protective effect of loganin on intestinal barrier in severely burned rats and explore the possible molecular mechanism.Methods:30 male SD rats were randomly divided into control group,burn group and loganin group,with 10 rats in each group.The rats in the burn group and loganin group were subjected to 30%TBSA III degree burn on the back,and the rats in the control group were subjected to sham burn.The rats in the loganin group were intragastrically administered with 5 mg loganin by gavage,the other two groups were given the same amount of normal saline by gavage,once daily for 7 days.After administration,the portal vein blood of mice was collected,and the intestinal permeability was detected by fluorescein isothiocyanate(FITC)-glucose fluorescence diagnostic tracer method.Western blotting was used to detect the expression of interleukin(IL)-1βand IL-6 in rat intestinal mucosa,and the expression of silent information regulatorl(SIRT1)and nuclear transcription factor-κB(NF-κB)p65 in rat ileum tissue.Results:Compared with the control group,the intestinal permeability of burn group and loganin group increased(P<0.05),the intestinal permeability of the loganin group was lower than that of the burn group(P<0.05).Compared with the control group,the levels of IL-1βand IL-6 in intestinal mucosa of the burn group and loganin group increased(P<0.05),and the loganin group was lower than the burn group(P<0.05).Compared with the control group,the expression of SIRT1 in ileal tissues of burn group and loganin group decreased(P<0.05),and the expression of NF-κB p65 increased(P<0.05).Compared with burn group,the expression of SIRT1 in ileal tissue of loganin group increased(P<0.05),and the expression of NF-κB p65 decreased(P<0.05).Conclusion:Loganin has protective effect on intestinal barrier in severely burned rats,and its mechanism may be related to mediating SIRT1/NF-κB signaling pathway and inhibiting inflammatory response.

关 键 词：烧伤 肠道屏障 肠道通透性 马钱苷 炎症 

分 类 号：R644[医药卫生—外科学]