1,5-AG在高糖代谢兔尸体玻璃体液中的变化  

Changes of 1,5-AG in Vitreous Humor of Rabbit Cadavers with Hyperglycemic Metabolism

作　　者：刘芳芳 吴慧 王威 谢英[1] LIU Fang-Fang;WU Hui;WANG Wei;XIE Ying(School of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,Sich-uan Province,China)

机构地区：[1]川北医学院基础医学与法医学院,四川南充637000

出　　处：《法医学杂志》2023年第1期13-17,共5页Journal of Forensic Medicine

基　　金：南充市市校合作科研专项资金资助项目(19SXHZ0197)。

摘　　要：目的了解1,5-脱水葡萄糖醇(1,5-anhydroglucitol,1,5-AG)在高糖代谢兔尸体玻璃体液中的浓度水平及变化特点,探讨1,5-AG在高糖代谢紊乱所致死亡的法医病理学中的鉴定价值。方法采用四氧嘧啶建立糖尿病高血糖兔模型,选取18只空腹血糖浓度≥13.80 mmol/L的糖尿病高血糖兔(实验组)和18只空腹血糖浓度≤6.10 mmol/L的健康兔(对照组),采用空气栓塞处死,即刻采集血液,于死后0、12、24、36 h采集玻璃体液,检测兔血液及玻璃体液中1,5-AG的质量浓度。结果实验组血糖浓度为(25.10±3.14)mmol/L;1,5-AG质量浓度在死亡即刻血浆中[(0.94±0.20)μg/mL]与玻璃体液中[(0.99±0.05)μg/mL]差异无统计学意义(P>0.05)。实验组各时间点玻璃体液中1,5-AG质量浓度低于相应对照组(P<0.05),实验组及对照组各时间点玻璃体1,5-AG质量浓度差异无统计学意义(P>0.05)。对照组和实验组兔血浆中1,5-AG质量浓度与血糖浓度均呈负相关(对照组r=-0.79,P<0.05;实验组r=-0.97,P<0.05)。结论玻璃体液可以替代血液作为检测1,5-AG的有效检材,兔玻璃体液中1,5-AG质量浓度在死后36 h内保持稳定,不受死后时间变化的影响。1,5-AG质量浓度明显下降,提示兔生前存在高血糖状态。Objective To investigate the concentration and change characteristics of 1,5-anhydroglucitol(1,5-AG)in the vitreous humor of rabbit cadavers with hyperglycemic metabolism,and to explore the value of 1,5-AG in forensic pathology identification of death caused by hyperglycemic metabolism disorders.Methods A diabetic hyperglycemic rabbit model was established by using alloxan.Eighteen rabbits with fasting glucose concentration≥13.80 mmol/L(experimental group)and 18 healthy rabbits with fasting glucose concentration≤6.10 mmol/L(control group)were selected.After death from air embolism.The blood samples were collected immediately,and vitreous humor samples were collected at 0 h,12 h,24 h and 36 h after death.The concentration of 1,5-AG in the blood and vitreous humor of rabbits was determined.Results The blood glucose concentration in the experimental group was(25.10±3.14)mmol/L.At the time of death,there was no significant difference in the concentration of 1,5-AG in the blood[(0.94±0.20)μg/mL]and in the vitreous humor(0.99±0.05μg/mL,P>0.05).The concentration of 1,5-AG in the vitreous humor of the experimental group was lower than that of the corresponding control group at all time points(P<0.05),and there was no significant difference betwwen 1,5-AG concentration in vitreous humor between earch time point in the experimental group and the control group(P>0.05).Correlation analysis showed that the concentration of 1,5-AG in blood was negatively correlated with blood glucose in both control group and experimental group(control group:r=-0.79,P<0.05;experimental group:r=-0.97,P<0.05).Conclusion Vitreous humor can replace blood as an effective test sample for 1,5-AG detection.The concentration of 1,5-AG in rabbit vitreous humor remains stable within 36 hours after death and is not affected by the change of postmortem interval.If the concentration of 1,5-AG decreases significantly,it indicates the existence of hyperglycemia in rabbits before death.

关 键 词：法医病理学 1 5-脱水葡萄糖醇 高血糖 玻璃体液 糖尿病 兔 

分 类 号：DF795.1[医药卫生—法医学]