NaCl胁迫对白籽南瓜RBCs生理特性的影响  

Effects of NaCl on Root Border Cells Genesis of Pumpkin Seedlings

作　　者：孙梦影 乔永旭 张永平 杨丹 李阮玲 张周 张莹莹 SUN Mengying;QIAO Yongxu;ZHANG Yongping;YANG Dan;LI Ruanling;ZHANG Zhou;ZHANG Yingying(College of Construction Engineering,Suqian College/Beijing-Hangzhou Grand Canal Ecological Civilization Research Center,Suqian Jiangsu 223800)

机构地区：[1]宿迁学院建筑工程学院/大运河文化带建设研究院宿迁分院生态文明研究中心,江苏宿迁223800

出　　处：《安徽农学通报》2023年第3期18-20,25,共4页Anhui Agricultural Science Bulletin

基　　金：宿迁市科技计划(创新能力建设)项目(M202001);宿迁学院大学生创新训练项目(2021XSJ082Y);宿迁学院产教融合型课程建设项目(2022CJRH23);宿迁学院课程思政示范课程建设项目(2021kskc30);宿迁学院人才引进科研启动基金项目(校2022XQT004)。

摘　　要：为了探究根系边缘细胞(root border cells,RBCs)在植物抗盐胁迫中的作用和机制,以白籽南瓜种子为试材,研究0、50、100、150、200和250 mmol/L NaCl对植株根长、RBCs数目、RBCs活率和RBCs黏胶层厚度的影响。结果表明,NaCl胁迫降低了植株根系的长度,随着NaCl浓度的增加,根系生长受到的抑制更明显;随着NaCl胁迫时间和浓度的增加,根系RBCs数目和活率也呈现不同程度的降低,其中200 mmol/L NaCl胁迫36 h的抑制效果最明显;但RBCs黏胶层厚度随NaCl胁迫浓度的增加而增加,200 mmol/L NaCl胁迫的RBCs黏胶层厚度最大。总之,NaCl胁迫对白籽南瓜根系的生长有一定的伤害作用,RBCs通过自身死亡和增加黏胶层厚度来减轻这种伤害。The effects of 0、50、100、150、200 and 250 mmol/L NaCl on plant root length,Root Border Cells(RBCs)number,RBCs viability and RBCs mucilage layer thickness taking the pumpkin seeds as material were investigated in order to explore the role and mechanism of RBCs in plant resistance to salt stress.The results showed that NaCl stress reduced the length of the root system,and the root growth was inhibited more obviously with the increasing of NaCl concentration.With the increasing of NaCl stress time and concentration,the number and viability of root RBCs showed a downward trend,especially 200 mmol/L NaCl stress for 36 h had the most obvious inhibitory effect.However,the thickness of the mucilage layer of RBCs increased with the increase of NaCl stress concentration,and the thickness of the mucilage layer of RBCs under 200 mmol/L NaCl stress was the largest.In conclusion,NaCl stress had a certain harmful effect on the root growth of the pumpkin,and RBCs alleviated the damage by dying themselves and increasing the thickness of the mucilage layer.

关 键 词：NACL胁迫 白籽南瓜 根系边缘细胞 黏胶层 

分 类 号：S642.9[农业科学—蔬菜学]