紫丁香苷对低剂量辐射小鼠生殖系统损伤的防护作用初探  

Protective effects of syringin against reproductive system injury induced by low dose radiation in mice

作　　者：胡昌坤 张雪梅 肖成荣 王宇光 马增春 高月[1,2] HU Changkun;ZHANG Xuemei;XIAO Chengrong;WANG Yuguang;MA Zengchun;GAO Yue(School of Basic Medical Sciences,Anhui Medical University,Hefei Anhui 230032,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;Trational Chinese Medicine College,3Guangdong Pharmaceutical University,Guangzhou Guangdong 510006,China)

机构地区：[1]安徽医科大学基础医学院,安徽合肥230032 [2]军事科学院军事医学研究院辐射医学研究所,北京100850 [3]广东药科大学中药学院,广东广州510006

出　　处：《中国药物警戒》2023年第3期277-281,共5页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(81873063);国家重点研发计划(2019YFC1604900);国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-C-202009)。

摘　　要：目的探索紫丁香苷对低剂量辐射导致小鼠生殖系统损伤的防护作用。方法通过对小鼠进行连续5 d的低剂量照射(0.12 Gy·d^(-1)×5 d累计0.6 Gy)建立低剂量辐射小鼠损伤模型,设立对照组、辐照组、低剂量给药组(紫丁香苷10 mg·kg^(-1))、高剂量给药组(紫丁香苷20 mg·kg^(-1)),照射结束后24 h和48 h分别通过酶联免疫吸附(ELISA)法测定小鼠睾丸内细胞因子如白介素-1β(IL-1β)、白介素-6(IL-6)、白介素-10(IL-10)、肿瘤坏死因子-α(TNF-α),以及睾酮含量,试剂盒测定睾丸内氧化应激水平如丙二醛(MDA)含量、超氧物歧化酶(SOD)活性、谷胱甘肽(GSH)含量变化。结果照射结束后24及48 h,相比于辐照组,紫丁香苷给药组能够明显调节小鼠睾丸内细胞因子紊乱,改善小鼠睾丸内氧化应激水平,恢复小鼠睾丸内睾酮含量水平。结论紫丁香苷能够改善低剂量辐射导致小鼠睾丸内细胞因子调节紊乱,有效降低小鼠生殖系统损伤,可作为低剂量辐射生殖系统损伤防护药物潜在研究对象。Objective To explore the protective effects of syringin against reproductive system injury induced by low dose radiation in mice.Methods A injury model was established after five consecutive days of low dose radiation(0.12 Gy·d^(-1)×5 d,cumulative 0.6 Gy).A control group(NC),radiation group(R),low dose administration group(syringin 10 mg·kg^(-1)),high dose administration group(syringin 20 mg·kg^(-1))were established.The contents of serum cytokines(IL-1β,IL-6,IL-10,and TNF-α)and testosterone in the mice 24 h and 48 h after irradiation were determined by ELISA,and the levels of oxidative stress(MDA content,SOD activity,and GSH content)in the testis were determined by the kit.Results 24 h and 48 h after irradiation,the cytokine disorders in the testis of mice were significantly regulated,the oxidative stress level improved,and the testosterone level restored in the syringin administration group compared with the irradiation group.Conclusion Syringin can improve cytokine regulation disorders induced by low dose radiation in mice,reduce the damage to the reproductive system in mice effectively,and might be used as a potential protective drug against reproductive system damage in case of low dose radiation.

关 键 词：紫丁香苷 低剂量辐射 小鼠 睾丸 防护作用 生殖系统 损伤 

分 类 号：R979.6[医药卫生—药品]