肉苁蓉苯乙醇总苷对胆汁淤积型肝病小鼠的预防保护作用  

Protective effects of phenylethanol glycosides of herba cistanche on mice with cholestatic liver disease

作　　者：曹琳 居博伟 冉峥 杨建华[3] 胡君萍[1] CAO Lin;JU Bowei;RAN Zheng;YANG Jianhua;HU Junping(College of Pharmaceutical Sciences,Xinjiang Medical University,Urumqi Xinjiang 830054,China;Department of Pharmacy,the Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi Xinjiang 830054,China;Department of Pharmacy,the First Affiliated Hospital of Xinjiang Medical University,Urumqi Xinjiang 830054,China)

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830054 [2]新疆医科大学第五附属医院药学部,新疆乌鲁木齐830054 [3]新疆医科大学第一附属医院药学部,新疆乌鲁木齐830054

出　　处：《中国药物警戒》2023年第3期286-291,共6页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(81860735)。

摘　　要：目的考察肉苁蓉苯乙醇总苷对α-萘异硫氰酸酯(α-naphthalene isothiocyanate,ANIT)诱导的小鼠胆汁淤积性肝病的预防保护作用。方法采用C57小鼠连续灌胃给予肉苁蓉苯乙醇总苷125、250、500 mg·kg^(-1)(肉苁蓉苯乙醇总苷低、中、高剂量组)和阳性药甘利欣100 mg·kg^(-1)7 d,每日给药1次,第5天给药后2 h单次灌胃给予ANIT 80 mg·kg^(-1)诱导造模,模型组小鼠等量灌胃生理盐水,其余各组小鼠继续用药干预2 d后收集小鼠血液与组织样本。检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、总胆红素(TBIL)、胆汁酸(TBA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平,观察肝脏组织病理学变化。结果与模型组相比,肉苁蓉苯乙醇总苷各组能够明显降低ANIT模型小鼠中ALT、AST、AKP、TBA、TBIL和MDA水平(P<0.05);肉苁蓉苯乙醇总苷中、高剂量组显著提高ALB、GSH-Px和SOD的活性(P<0.05)。结论肉苁蓉苯乙醇总苷可能通过改善细胞膜通透性和消除自由基氧化,从而发挥对ANIT所诱导的胆汁淤积性肝病小鼠的预防保护作用。Objective To investigate the protective effect of phenylethanol glycosides from Cistanche deserticola againstα-naphthalene isothiocyanate(ANIT)-induced cholestatic liver disease in mice.Methods C57 mice were orally administered with 125,250,500 mg·kg^(-1)of phenylethanol glycosides from Cistanche deserticola and 100 mg·kg^(-1)of diammonium glycyrrhizinate for seventeen consecutive days,once daily.80 mg·kg^(-1)of ANIT was administered by a single gavage two hours after administration on the fifth day to induce a model.Blood and tissue samples of mice were collected after two days of continuous intervention.Levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),albumin(ALB),alkaline phosphatase(AKP),lactate dehydrogenase(LDH),total bilirubin(TBIL),bile acid(TBA),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)and malondialdehyde(MDA)were detected,while the histopathological changes of the liver were observed.Results Compared with the model group,the levels of ALT,AST,AKP,TBA,TBIL and MDA in ANIT model mice were significantly decreased in each group of phenylethanol glycosides from Cistanche deserticola(P<0.05).The activities of ALB,GSH-Px and SOD were significantly increased in the medium and high dose groups(P<0.05).Conclusion Cistanche deserticola may help protect against ANIT-induced cholestatic liver disease by improving cell membrane permeability and eliminating free radical oxidation.

关 键 词：肉苁蓉 苯乙醇总苷 Α-萘异硫氰酸酯 胆汁淤积 小鼠 

分 类 号：R961[医药卫生—药理学] R975[医药卫生—药学]