金槐精制粉对高脂血症大鼠血管内皮过氧化和炎症反应的影响  

Effect of Jinhuai Refined Powder on Vascular Endothelial Peroxidation and Inflammation in Hyperlipidemia Rats

作　　者：庞大承 张硕 牟天龙 王云飞 邱采娴 罗佳雨 陈怀玉 白涵 田甜 潘彦舒 PANG Dacheng;ZHANG Shuo;MOU Tianlong;WANG Yunfei;QIU Caixian;LUO Jiayu;CHEN Huaiyu;BAI Han;TIAN Tian;PAN Yanshu(College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China;Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China)

机构地区：[1]北京中医药大学中医学院,北京100029 [2]北京中医药大学第三临床医学院,北京100029 [3]北京中医药大学东方医院,北京100078

出　　处：《世界中医药》2023年第3期309-313,共5页World Chinese Medicine

基　　金：国自然青年科学基金项目(82204963);2019年度北京中医药大学校级科研纵向发展基金项目(2019-ZXFZJJ-064);2020年度北京中医药大学基本科研业务费项目重点攻关项目(2020-JYB-ZDGG-017)。

摘　　要：目的:通过给予不同剂量的金槐精制粉,观察其对高脂血症(HLP)大鼠血管内皮过氧化和炎症反应的影响,从而探究金槐精制粉对血管内皮的保护效果。方法:实验动物按体质量大小均匀分为正常组、模型组、金槐高、中、低剂量组和曲克芦丁组,正常组喂以普通清洁级饲料,其余各组喂以高脂饲料,喂养30 d;成模后,正常组和模型组按照0.9%生理盐水10 mL/(kg·d)灌胃,金槐高、中、低剂量组分别按照102.45 mg/(kg·d)、83.65 mg/(kg·d)、68.30 mg/(kg·d)灌胃给药,曲克芦丁组按照39.70 mg/(kg·d)灌胃给药,14 d后,腹主动脉取血;采用酶联免疫吸附试验法(ELISA)检测大鼠血清中白细胞介素-6(IL-6)、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)、超氧化物歧化酶(SOD)的含量。结果:与正常组比较,模型组血清中IL-6、CRP、TNF-α水平明显升高(均P<0.05),与模型组比较,金槐高、中剂量组和曲克芦丁组IL-6均有所下降(均P<0.05),各组CRP均有所下降(均P<0.05),金槐高剂量组和曲克芦丁组TNF-α有所下降(均P<0.05);与正常组比较,模型组血清中SOD明显下降,MDA明显升高(均P<0.05),与模型组比较,金槐高、中、低剂量组和曲克芦丁组SOD均有上升(均P<0.05),金槐高剂量组和曲克芦丁组MDA均有下降(均P<0.05)。结论:金槐精制粉可以改善HLP大鼠血管内皮过氧化,抑制炎症反应,对血管内皮具有一定的保护作用,并呈一定的剂量依赖性,以高剂量的改善较为明显。Objective:To observe the effects of Jinhuai refined powder on vascular endothelial peroxidation and inflammation in hyperlipidemia rats by administering different doses of Jinhuai refined powder to rats,so as to explore the protective effect of Jinhuai refined powder on vascular endothelium.Methods:By the body weight,the experimental animals were evenly divided into the normal group,model group,Jinhuai high-dose,medium-dose and low-dose groups and troxerutin group.The normal group was fed with ordinary clean grade diet,and other groups were fed with high-fat diet for 30 days.After model formation,the normal group and model group were given 0.9%normal saline 10 mL/(kg·d)by gavage,Jinhuai high-dose medium-dose and low-dose groups were given 102.45 mg/(kg·d),83.65 mg/(kg·d)and 68.30 mg/(kg·d)drugs respectively by gavage,and the troxerutin group was given 39.70 mg/(kg·d)drugs by gavage.Fourteen days later,blood was collected from the abdominal aorta;the serum levels of IL-6,CRP,TNF-α,MDA and SOD were determined by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the normal group,the serum levels of IL-6,CRP and TNF-αin the model group significantly increased(P<0.05);compared with the model group,the serum levels of IL-6 in Jinhuai high-dose and medium-dose groups and troxerutin group decreased(P<0.05);the serum levels of CRP in each group decreased(P<0.05);the serum levels of TNF-αin the Jinhuai high-dose group and troxerutin group decreased(P<0.05);compared with the normal group,the serum levels of SOD in the model group significantly decreased and those of MDA significantly increased(P<0.05);compared with the model group,the serum levels of SOD in Jinhuai high-dose,medium-dose and low-dose groups and troxerutin group increased(P<0.05),and those of MDA in the Jinhuai high-dose group and troxerutin group decreased(P<0.05).Conclusion:Jinhuai refined powder can improve the peroxidation of vascular endothelium in hyperlipidemia rats,inhibit the inflammatory reaction,and has a certain protective eff

关 键 词：金槐精制粉 曲克芦丁 高脂血症 抗氧化 抗自由基 炎症反应 高脂血症大鼠 血管保护作用 

分 类 号：R282[医药卫生—中药学] R283[医药卫生—中医学]