丹参有效成分最佳配伍对载脂蛋白E^(-/-)小鼠血管外膜成纤维细胞增殖的影响  

Effects of Optimal Compatibility of Active Components of Salvia Miltiorrhiza on Proliferation of Vascular Adventitial Fibroblasts in ApoE^(-/-)Mice

作　　者：张帅[1] 安胜军[1] 崔清卓 周勇杰 武若愚 宁浚达 冯慧敏[1] 张宇[1] 徐红俊[1,2] ZHANG Shuai;AN Shengjun;CUI Qingzhuo;ZHOU Yongjie;WU Ruoyu;NING Junda;FENG Huimin;ZHANG Yu;XU Hongjun(Hebei University of Chinese Medicine,Hebei Engineering Center of Plant Bioreactor Preparation Technology,Shijiazhuang 050200,China;College of Traditional Chinese and Western Medicine,Hebei University of Chinese Medicine,Shijiazhuang 050200,China)

机构地区：[1]河北中医学院河北省植物生物反应器制备技术工程实验室,石家庄050200 [2]河北中医学院中西医结合学院,石家庄050200

出　　处：《世界中医药》2023年第3期320-323,共4页World Chinese Medicine

基　　金：河北省中医药管理局科研计划项目(2019073);河北省省级科技计划项目(213777100D);河北中医学院校内青年基金项目(QNZ2018010)。

摘　　要：目的:探讨丹参水溶性有效成分最佳配伍(SABP)对载脂蛋白E^(-/-)小鼠体内和体外血管外膜成纤维细胞(VAFs)增殖的干预作用。方法:对原代培养C57BL/6J小鼠、载脂蛋白E^(-/-)小鼠VAFs,体外分别给予正常培养基、SABP、瑞舒伐他汀。将载脂蛋白E^(-/-)小鼠分为模型组、SABP组、瑞舒伐他汀组,C57BL/6J小鼠作正常对照组,原代培养给药后的各组小鼠VAFs,采用CCK-8法检测VAFs的增殖情况。结果:体外和体内结果均显示,SABP和瑞舒伐他汀均明显抑制了载脂蛋白E^(-/-)小鼠VAFs的增殖,且SABP作用更显著。结论:SABP对载脂蛋白E^(-/-)小鼠VAFs的增殖有明显的抑制作用,有望改善血管重构,成为治疗心血管疾病替代药物。Objective:To explore the intervention effect of the optimal compatibility(SABP)of the water-soluble active ingredients of Salvia miltiorrhiza on the proliferation of ApoE^(-/-)mouse vascular adventitia fibroblasts(VAFs)in vivo and in vitro.Methods:The VAFs of primarily cultured C57BL/6J mice and ApoE^(-/-)mice were given normal medium,SABP and rosuvastatin(RC)in vitro.ApoE^(-/-)mice were divided into the model group,SABP group and RC group,and C57BL/6 mice were used as the normal control group.After primary culture and administration,the proliferation of VAFs was detected by CCK-8 method.Results:In vitro and in vivo results showed that SABP and RC significantly inhibited the proliferation of VAFs in ApoE^(-/-)mice,of which SABP had a more significant effect.Conclusion:SABP can significantly inhibit the proliferation of VAFs in ApoE^(-/-)mice,which is expected to improve vascular remodeling and become a substitute drug for cardiovascular diseases.

关 键 词：丹参 丹酚酸A 丹酚酸B 丹参素 原儿茶醛 血管外膜成纤维细胞 增殖 血管重塑 

分 类 号：R285.5[医药卫生—中药学]