桃红饮减轻ApoE^(-/-)小鼠动脉粥样硬化并发非酒精性脂肪肝氧化应激损伤和纤维化的作用研究  

Effect of Taohongyin decoction on oxidative stress injury and fibrosis of atherosclerosis with non-alcoholic fatty liver in ApoE^(-/-)mice

作　　者：冯骁腾 杜敏 张一凡 李斯锦 丁洁 王佳柔 王怡茹 刘萍[1] FENG Xiao-teng;DU Min;ZHANG Yi-fan;LI Si-jin;DING Jie;WANG Jia-rou;WANG Yi-ru;LIU Ping(Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai,200032,China)

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《时珍国医国药》2023年第1期1-5,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(82074200,81873117)。

摘　　要：目的探究活血通络法代表方桃红饮对动脉粥样硬化并发非酒精性脂肪肝炎小鼠肝组织炎症损伤和纤维化的影响。方法野生型雄性C57/B6J小鼠10只为空白组,ApoE^(-/-)/C57/B6J小鼠40只,随机分为模型组、阿托伐他汀组和中药治疗组(桃红饮高、中、低剂量组)。模型组、阿托伐他汀组和中药治疗组给予西方高脂饮食造模12周,阿托伐他汀(5mg/kg)和中药组(11.54g/kg,5.77g/kg,2.89g/kg)以灌胃给药,空白组和模型组以灭菌纯水灌胃12周,检测血清ALT、AST、TRIG、TC、LDL,以及肝组织SOD、MDA和GSH水平;HE染色评估肝组织形态和炎性浸润、Masson染色评估肝组织纤维化程度、肝脏免疫荧光染色评估淋巴管数量和形态。结果与模型组相比,干预组不同程度减低了血清TRIG、TC、LDL的水平(P<0.05),增强了肝脏SOD、GSH的活性(P<0.01),降低MDA(P<0.01),减轻了肝脏脂肪变性及纤维化进展,其中高剂量组作用最为显著。结论通过活血通络法代表方桃红饮治疗可以减缓动脉粥样硬化及并发的NAFLD进展,剂量与疗效显示正相关,这种作用可能与调控肝脏淋巴管生成途径有关。Objective To investigate the effect of Taohongyin decoction,a representative recipe of Huoxuetongluo theory,on inflammatory injury and fibrosis of liver tissue in mice with atherosclerosis with non-alcoholic steatohepatitis.Methods 10 wild-type male C57/B6j mice were used as the blank group and 40 ApoE^(-/-)/C57/B6j mice were randomly divided into model group,atorvastatin group and traditional Chinese medicine treatment group(the high,medium and low dose groups of Taohongyin decoction).The model group,atorvastatin group and traditional Chinese medicine treatment group were kept with western high-fat diet for 12 weeks,atorvastatin(5mg/kg)and traditional Chinese medicine group(11.54g/kg,5.77g/kg,2.89g/kg)were given by gavage,and the blank group and model group were given sterilized pure water by gavage for 12 weeks.Serum ALT,AST,TRIG,TC,and liver SOD,MDA and GSH were measured.HE staining was used to evaluate the morphology and inflammatory infiltration of liver tissue,Masson staining was used to evaluate the degree of liver fibrosis,and liver immunofluorescence staining was used to evaluate the number and morphology of lymphatic vessels.Results Compared with the model group,the drug group reduced the levels of serum trig,TC and LDL in varying degrees(P<0.05),enhanced the activities of liver SOD and GSH(P<0.01),reduced MDA(P<0.01),and reduced the progression of hepatic steatosis and fibrosis,among which the effect of high-dose group was the most significant.Conclusion The treatment of Taohongyin decoction represented by the method of promoting blood circulation and dredging collaterals can significantly slow down the progress of atheroselerosis and complicated NAFLD.The dose is positively correlated with the curative effect.This effect may be related to the regulation of hepatic lymphangiogenesis.

关 键 词：非酒精性脂肪肝 桃红饮 动脉粥样硬化 炎症 淋巴管 

分 类 号：R285.5[医药卫生—中药学]