基于GC-MS代谢组学的胆木糖浆抗H1N1甲型流感病毒性肺炎研究  

Study on the Nauclea Officinalis Syrup againstinfluenza A virus(H1N1)pneumonia based on GC-MS metabonomics

作　　者：沈存思 钱文娟 林丽丽 陈古月 项莹颖 张正光[2] 谢彤 纪建建 徐建亚[2] 汪受传 单进军 SHEN Cun-si;QIAN Wen-juan;LIN Li-li;CHEN Gu-yue;XIANG Ying-ying;ZHANG Zheng-guang;XIE Tong;JI Jian-jian;XU Jian-ya;WANG Shou-chuan;SHAN Jin-jun(Jiangsu Key Laboratory of Pediatric Respiratory Disease,Institute of Pediatics,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing,210023,China;School of Medicine﹠Holistic Integrative Medicine,Nanjing University of Chinese Medicine,Nanjing,210023,China)

机构地区：[1]江苏省儿童呼吸疾病(中医药)重点实验室,南京中医药大学附属医院,中医儿科学研究所,江苏南京210023 [2]南京中医药大学医学·整合医学学院,江苏南京210023

出　　处：《时珍国医国药》2023年第1期206-211,共6页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81904029);南京中医药大学自然科学基金青年项目(NZY81904029);江苏省高等学校自然科学研究面上项目(19KJB360010)。

摘　　要：目的采用非靶标代谢组学方法探讨胆木糖浆干预H1N1甲型流感病毒性肺炎相关代谢网络变化及可能的作用机制。方法随机将ICR小鼠分成四组:正常组、H1N1肺炎模型组、阳性药达菲组、胆木糖浆组。模型组以H1N1病毒滴鼻造模,给药组以胆木糖浆灌胃治疗,正常组以生理盐水灌胃作为对照。实验结束后取肺组织样本,采用GC-MS代谢组学技术分析小鼠肺组织中的差异性代谢产物(FDR<0.05,Fold Change>1.5)并寻找其潜在的代谢通路。结果主成分分析(PCA)结果显示各组间小鼠肺组织的代谢轮廓可明显区分,筛选出21种潜在差异性代谢产物在模型组出现不同趋势改变,胆木糖浆组具有明显回调作用。代谢通路富集分析进一步提示胆木糖浆主要影响了嘌呤、嘧啶及磷酸戊糖途径等代谢相关通路发挥治疗H1N1肺炎的作用。结论胆木糖浆主要可通过调节机体嘌呤、嘧啶及磷酸戊糖代谢途径及其相关的代谢产物发挥治疗H1N1肺炎的作用。Objective The method of non-target metabonomics was used to explore the changes of metabolic network related to the intervention of Nauclea Officinalis Syrup on influenza A virus(H1N1)pneumonia and its possible mechanism.Methods ICR mice were randomly divided into four groups:normal group,H1N1 pneumonia model group,positive drug Tamiflu group,and-Nauclea Officinalis Syrup group.The model group was modeled with H1N1 virus nasal drip,the treatment group was treated with Nauclea Officinalis Syrup via gavage,and the normal group was treated with saline via gavage as control.After the experiment,lung tissue sampleswere taken,and GC-MS metabolomics technology was used to analyze the differential metabolites(FDR<0.05,Fold Change>1.5)in the lung tissues of mice and search for their potential metabolic pathways.Results Principal component analysis(PCA)results showed that the metabolic profile of mouse lung tissues couldbe clearly distinguished among different group.21 potential different metabolites were screened and showed different trends in the model group and the Nauclea Officinalis Syrup group had a significant callback effect.Metabolic pathway enrichment analysis further suggested that Nauclea Officinalis Syrup mainly affect the metabolic related pathways such as purine,pyrimidine and pentose phosphate pathways to play the role of treating HIN1 pneumonia.Conclusion Nauclea Officinalis Syrup may treat H1N1 pneumonia mainly by regula-ting the metabolic pathways of purine,pyrimidine and pentose phosphate and its related metabolites.

关 键 词：GC-MS 代谢产物 胆木糖浆 甲型H1N1 肺炎 

分 类 号：R256[医药卫生—中医内科学] R254[医药卫生—中医学]