津力达联合通心络通过Nrf-2/HO-1信号通路对高糖诱导的人脐静脉内皮细胞损伤的干预作用  

Intervention effect of Jinlida combined with Tongxinluo on human umbilical venous endothelial cells damage induced by hyperglycemia via Nrf-2/HO-1 signaling pathway

作　　者：郑文丽 魏艳婷 位庚[1] ZHENG Wenli;WEI Yanting;WEI Geng(Department of Pharmacy,Shijiazhuang Second Hospital,Hebei Provincial Key Laboratory of Basic Medicine for Diabetes Mellitus,Shijiazhuang Technology Innovation Center for Precision Medicine for Diabetes Mellitus,Shijiazhuang 050500,Hebei,China;Department of Pharmacy,Shijiazhuang Fourth Hospital,Shijiazhuang 050035,Hebei,China)

机构地区：[1]石家庄市第二医院药剂科、河北省糖尿病基础医学研究重点实验室、石家庄市糖尿病精准诊疗技术创新中心,河北省石家庄市050500 [2]石家庄市第四医院药剂科,河北省石家庄市050035

出　　处：《广西医学》2023年第4期435-442,共8页Guangxi Medical Journal

基　　金：河北省中医药类科研计划课题(2020275)。

摘　　要：目的探讨津力达联合通心络通过核因子E2相关因子2(Nrf-2)/血红素氧合酶1(HO-1)信号通路对高糖诱导的人脐静脉内皮细胞(HUVECs)损伤的干预作用。方法将HUVECs分成正常对照组、模型组、津力达组、通心络组、联合用药组,正常对照组细胞采用含5.5 mmol/L D-葡萄糖的DMEM培养,模型组、津力达组、通心络组、联合用药组细胞采用含30 mmol/L D-葡萄糖的DMEM培养,津力达组加入200 mg/L的津力达贮存液,通心络组加入100 mg/L的通心络贮存液,联合用药组加入200 mg/L的津力达贮存液和100 mg/L的通心络贮存液。采用CCK-8法检测各组细胞活力,采用克隆形成实验检测各组细胞的克隆形成数量,采用Transwell检测细胞侵袭能力,采用流式细胞仪检测各组细胞凋亡率,采用划痕实验检测各组细胞迁移率,采用ELISA检测各组细胞的炎症因子水平,检测各组细胞的氧化应激相关指标水平,采用Western blot检测各组细胞的Nrf-2、HO-1蛋白表达量。结果与正常对照组相比,模型组细胞的活力、克隆形成数量、侵袭细胞数量、细胞迁移率均降低,细胞凋亡率升高,细胞间黏附分子(ICAM-1)、肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1β、IL-6、丙二醛、乳酸脱氢酶(LDH)水平均升高,超氧化物歧化酶(SOD)、一氧化氮、一氧化氮合成酶(NOS)水平及Nrf-2、HO-1蛋白表达量均降低(均P<0.05);与模型组相比,津力达组、通心络组和联合用药组细胞的活力、克隆形成数量、侵袭细胞数量、细胞迁移率均升高,细胞凋亡率均降低,ICAM-1、TNF-α、IL-1β、IL-6、丙二醛、LDH水平均降低,SOD、一氧化氮、NOS水平及Nrf-2、HO-1蛋白表达量均升高,且联合用药组上述指标均优于津力达组和通心络组(均P<0.05)。结论津力达和通心络均通过抑制炎症反应和氧化应激反应来减轻高糖所致的HUVECs损伤,且联合用药效果更好,其机制可能与激活Nrf-2/HO-1信号通路有关。Objective To explore the intervention effect of Jinlida combined with Tongxinluo on human umbilical venous endothelial cells(HUVECs)damage induced by hyperglycemia via nuclear factor E2-related factor 2(Nrf-2)/heme oxygenase 1(HO-1)signaling pathway.Methods HUVECs were assigned to normal control group,model group,Jinlida group,Tongxinluo group,or combined medication group.Cells in the normal control group was cultured with DMEM containing 5.5 mmol/L D-glucose,cells in the model group,Jinlida group,Tongxinluo group,and combined medication group were cultured with DMEM containing 30 mmol/L D-glucose,and then the Jinlida group was added with 200 mg/L Jinlida stock solution,and the Tongxinluo group was added with 100 mg/L Tongxinluo stock solution,as well as the combined medication group was added with 200 mg/L Jinlida stock solution and 100 mg/L Tongxinluo stock solution.Cell viability was detected in various groups by the CCK-8 method,the number of clones formed in various groups was detected by using clone formation assay,cell invasive ability was detected by the Transwell method,the apoptosis rate in various groups was detected by employing flow cytometry,the cell migration rate in various groups was detected by the application of scratch assay,the ELISA was used to detect the expressions of inflammatory factors of cells in various groups,and the levels of oxidative stress-related indices were detected in various group,as well as the Western blot was used for detecting protein expressions of Nrf-2 and HO-1 of cells in various groups.Results Compared with the normal control group,the model group obtained decreased cell viability,number of clones formed,number of invasive cells,and cell migration rate,while an elevated rate of cell apoptosis,and elevated levels of intercellular adhesion molecule 1(ICAM-1),tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-6,malondialdehyde,and lactic dehydrogenase(LDH),as well as decreased levels of superoxide dismutase(SOD),nitric oxide,nitric oxide synthase(NOS),and decrease

关 键 词：人脐静脉内皮细胞 高糖损伤 津力达 通心络 核因子E2相关因子2 血红素氧合酶1 炎症因子 氧化应激 

分 类 号：R587.1[医药卫生—内分泌]