盾叶秋海棠叶片高频再生体系的建立  

Establishment of high-frequency regeneration system of Begonia peltatifolia from leaves

作　　者：陈英转 孟新亚 宋希强[1] 田代科[2] 周韬 CHEN Yingzhuan;MENG Xinya;SONG Xiqiang;TIAN Daike;ZHOU Tao(Key Laboratory of Biology of Flower Resources of Hainan Province/ollege of Forestry,Hainan University,Haikou 570228,China;Shanghai Chenshan Botanical Garden/Chenshan Plant Science Research Center of CAS Center for Excellence in Molecular Plant Sciences/Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai 201602,China)

机构地区：[1]海南省热带特色花木资源生物学重点实验室/海南大学林学院,海口570228 [2]上海辰山植物园/中国科学院分子植物科学卓越创新中心辰山植物科研中心/上海市资源植物功能基因组学重点实验室,上海201602

出　　处：《植物生理学报》2023年第1期165-171,共7页Plant Physiology Journal

基　　金：海南省重点研发项目(ZDYF2020099和ZDYF2020046)。

摘　　要：盾叶秋海棠(Begonia peltatifolia)利用叶片扦插繁殖困难,插穗很少能再生不定芽。为了更好地保护和利用该种资源,以其叶片作为外植体建立高效的组培体系。结果表明:最适用于盾叶秋海棠叶片愈伤组织和不定芽诱导的培养基均为MS+6-BA 1.00 mg·L^(-1)+NAA 0.15 mg·L^(-1),诱导率分别高达97.67%和71.00%;不定芽增殖最适培养基为MS+6-BA 1.25 mg·L^(-1)+NAA 0.05 mg·L^(-1),增殖系数达20.10;不定芽生根最适培养基为1/2MS+NAA 0.50 mg·L^(-1)(单芽)和1/2MS+NAA 0.10 mg·L^(-1)(丛芽),生根率分别为91.11%和100.00%。丛芽芽色深绿、根系健壮,移栽成活率达到90.63%以上。It is difficult to propagate Begonia peltatifolia by leaf cuttings which rarely regenerate shoots. For better protection and utilization of this species, its leaves are used as explants for efficient in vitro propagation. The results indicated that the optimum callus and shoots induction medium for the leaves were MS+6-BA 1.00 mg·L^(-1)+NAA 0.15 mg·L^(-1), with 97.67% and 71.00% induction rate. The proliferation coefficient of clustered shoots reached 20.10 in the MS+6-BA 1.25 mg·L^(-1)+NAA 0.05 mg·L^(-1)as optimum medium. The optimum rooting medium for clustered shoots was 1/2MS+NAA 0.50 mg·L^(-1)(single shoot) and 1/2MS+NAA 0.10 mg·L^(-1)(cluster shoots). The rooting rate of them was 91.11% and 100.00%, respectively.The shoots in the shoot-cluster state were dark green, and the roots were robust. After being transplanted,about 90.63% of plantlets survived.

关 键 词：盾叶秋海棠 叶片外植体 组织培养 种质资源保护 

分 类 号：S682.19[农业科学—观赏园艺]