白木香AsOMT1基因的克隆与表达分析  

Cloning and expression analysis of AsOMT1 gene in Aquilaria sinensis

作　　者：杨艳 朱家红[2,3] 曾军[2,3] 梅文莉[2,3] 戴好富[2,3] 彭世清[2,3] YANG Yan;ZHU Jiahong;ZENG Jun;MEI Wenli;DAI Haofu;PENG Shiqing(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163000,China;Key Laboratory of Biology and Genetic Resources of Tropical Crops,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province,Hainan Institute for Tropical Agricultural Resources,Haikou 571101,China;Hainan Engineering Research Center of Agarwood,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163000 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室/海南热带农业资源研究院海南省热带农业生物资源保护与利用重点实验室,海口571101 [3]中国热带农业科学院热带生物技术研究所,海南省沉香工程技术研究中心,海口571101

出　　处：《植物生理学报》2023年第1期200-208,共9页Plant Physiology Journal

基　　金：海南省自然科学基金(320RC713);国家自然科学基金(32171824、32171747和31870668)。

摘　　要：O-甲基转移酶(O-methyltransferase, OMT)是植物中调控次生代谢产物生物合成的一类关键酶,主要功能是催化酚类物质发生氧甲基化反应。本研究在白木香(Aquilaria sinensis)转录组中筛选到一个OMT编码基因AsOMT1,该基因与2-(2-苯乙基)色酮合成关键酶基因(PECPS)表达高度正相关。AsOMT1基因全长1 456 bp,包含一个1 074 bp的完整开放阅读框,编码357个氨基酸;氨基酸序列比对和系统进化聚类分析发现该As OMT1属于咖啡酸O-甲基转移酶亚家族。AsOMT1基因启动子区存在多个激素和胁迫应答元件;实时荧光定量PCR结果显示,伤害处理和乙烯处理均能显著上调AsOMT1的表达,推测其可能参与了沉香2-(2-苯乙基)色酮的生物合成。Plant O-methyltransferases(OMT) is one of the key enzymes in the regulation of secondary metabolites biosynthesis, which methylate the oxygen atom of phenolic compounds. AsOMT1 was screened by analyzing the transcriptome of Aquilaria sinensis, the expression of which is positively correlated with diarylpentanoid-producing polyketide synthase gene(PECPS). The full length of AsOMT1 is 1 456 bp with a 1 074 bp opening reading frame(ORF) and encoding a 375 amino acids protein. The amino acid sequence alignment results and phylogenetic analysis showed that AsOMT1 belongs to coffee acid O-methyltransferase types. Several responsive elements in response to hormones and stress are found in the promoter region of AsOMT1. Real-time PCR results showed that expression of AsOMT1 was significantly increased by wounding and ethephon treatment. These results indicated that AsOMT1 may be involved in the biosynthesis of 2-(2-phenethyl)chromone in agarwood.

关 键 词：白木香 O-甲基转移酶 AsOMT1 生物信息学 表达分析 

分 类 号：Q943.2[生物学—植物学]