黄瓜DME基因家族的全基因组鉴别及转录分析  

Genome-wide identification and transcriptional analysis of DME gene family in cucumber

作　　者：赵振翔 敖文红 王新法 卢琳 罗未蓉 孙涌栋 ZHAO Zhenxiang;AO Wenhong;WANG Xinfa;LU Lin;LUO Weirong;SUN Yongdong(School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,Henan 453003,China;Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement,Xinxiang,Henan 453003,China;School of Information Engineering,Henan Institute of Science and Technology.Xinxiang.Henan 453003,China)

机构地区：[1]河南科技学院园艺园林学院,河南新乡453003 [2]河南省园艺植物资源利用与种质创新工程研究中心,河南新乡453003 [3]河南科技学院信息工程学院,河南新乡453003

出　　处：《植物生理学报》2023年第1期209-218,共10页Plant Physiology Journal

基　　金：河南省重点研发与推广专项(212102110130);新乡市重点科技攻关项目(GG2019013)。

摘　　要：DEMETER (DME)是DNA去甲基化所需要的一种糖基化酶,对植物生长发育和逆境响应具有重要的调控作用。本文对黄瓜CsDME家族成员的基因结构、进化特征及转录模式进行了分析。结果表明,在黄瓜基因组中共鉴别了8个CsDMEs基因,命名为CsDME1~8。系统进化树分析将黄瓜CsDMEs家族成员划分为4个亚族,且黄瓜CsDME与西瓜ClDME的进化关系较近。顺式作用元件分析发现, CsDMEs启动子序列含有光、植物激素、胁迫响应和生长发育相关调控元件,说明CsDMEs可能参与了植物生长发育和逆境胁迫响应。组织特异性表达分析显示, CsDMEs与植物生长发育有关,且在不同的组织部位发挥的生物学功能不同。低温和NaCl胁迫下基因转录模式表明, CsDMEs可能受到低温和NaCl胁迫调控。该结果为深入研究CsDMEs在黄瓜生长发育及响应非生物胁迫中的生物学功能提供了参考。DEMETER(DME) is a glycosylase enzyme required for DNA demethylation and has an important regulatory role in plant growth and development and stress response. In this study, we analyzed the gene structure, evolutionary characteristics and expression patterns of CsDME family members in cucumber(Cucumis sativus). The results showed that a total of 8 CsDMEs were identified in the cucumber genome,and named CsDME1-8. CsDMEs were divided into 4 group based on phylogenetic analysis, and CsDME shared a closer genetic relationship with ClDME. Cis-acting element analysis revealed that the promoter sequences of CsDMEs included light, phytohormone, stress response and growth and development elements, impling that CsDMEs might be involved in the regulation of growth and development and stress response. Tissue-specific expression showed that CsDMEs were associated with plant growth and development and performed different biological functions in different tissues. Gene expression patterns indicated that CsDMEs might be regulated by low temperature and NaCl stresses. These results could be helpful for further exploration of the biological functions of CsDMEs in the growth and development and response to abiotic stress.

关 键 词：黄瓜 CsDME 生物信息学 基因表达 非生物胁迫 

分 类 号：S642.2[农业科学—蔬菜学]