小尾寒羊与双乾肉羊的瘤胃菌群差异分析  

Analysis on the Difference of Ruminal Microflora between Small-Tail Han Sheep and Shuangqian Mutton Sheep

作　　者：彭洪洋 李玉梅[1] 赵中利[2] 张英[1] 白春艳[1] 闫守庆[1] 马惠海[2] PENG Hongyang;LI Yumei;ZHAO Zhongli;ZHANG Ying;BAI Chunyan;YAN Shouqing;MA Huihai(College of Animal Science,Jilin University,Changchun 130062;Institute of Animal Husbandry and Veterinary,Jilin Academy of Agricultural Sciences,Gongzhuling 136100,China)

机构地区：[1]吉林大学动物科学学院,长春130062 [2]吉林省农业科学院畜牧兽医研究所,吉林公主岭136100

出　　处：《东北农业科学》2023年第1期50-53,107,共5页Journal of Northeast Agricultural Sciences

基　　金：吉林省科技攻关项目(20190301006NY);吉林省畜牧业管理局畜禽遗传资源多胎肉羊种群选育与开发项目(2021);国家现代农业产业技术体系项目(CARS-38)。

摘　　要：本试验旨在研究小尾寒羊(STH)及双乾肉羊(SQ)瘤胃微生物区系特征及品种间差异。随机挑选出生日期相近、初体重相近、健康的小尾寒羊和双乾肉羊各6只,采用16S rDNA扩增子测序方法,探究两个绵羊品种之间瘤胃微生物的差异,预测其微生物功能。结果表明,SQ组瘤胃菌群多样性和丰富性均高于STH组;主坐标分析和非加权组平均法聚类分析显示组间具有差异性。两个品种绵羊瘤胃菌群在门水平上组成相似,优势菌门均为拟杆菌门、厚壁菌门及变形菌门。对差异菌群进行KEGG功能预测,结果显示,双乾肉羊瘤胃微生物功能大部分高于小尾寒羊。从上述分析来看,双乾肉羊瘤胃菌群种类及丰度均高于小尾寒羊,这可能导致了双乾肉羊饲料利用率更高。The purpose of this experiment is to investigate the ruminal microflora characteristics and differences between Small-tail Han sheep(STH)and Shuangqian mutton sheep(SQ).6 STH and 6 SQ were randomly selected,which were similar birth date,initial body weight and good health.The 16S rDNA amplicon sequencing method was applied to explore the microbial differences between the rumen of two breeds and predict the microbial function.The results indicated that the bacterial diversity and richness of rumen of Shuangqian mutton sheep are higher than those of Small-tail Han sheep.The principal coordinate analysis(PCoA)and UPGMA cluster analysis showed that the bacteria community in two groups was different.The composition of rumen microflora of the two breeds of sheep is similar at the phylum level and dominant phyla were Bacteroidetes,Firmicutes and Proteobacteria.The results of KEGG pathway analysis revealed that the rumen microbial function of SQ was mostly higher than that of STH.Based on the above analysis,the species and abundance of ruminal microflora of SQ were greater compared with STH,which could lead to higher feed utilization rate of SQ.

关 键 词：小尾寒羊 瘤胃微生物 16S rDNA 菌群结构 

分 类 号：S826[农业科学—畜牧学]