藏药抱茎獐牙菜HPLC指纹图谱及10个成分含量测定研究  

Study on the chromatographic fingerprint and 10 components of Tibetan medicine Swertia franchetiana H.Smith by HPLC

作　　者：李维业 刘学良 刘安平 苏媛 杨增亮 韩达斌 石春兰 徐文胜 刘海青 LI Wei-ye;LIU Xue-liang;LIU An-ping;SU Yuan;YANG Zeng-liang;HAN Da-bin;SHI Chun-lan;XU Wen-sheng;LIU Hai-qing(Xining Center for Food and Drug Inspection&Testing,Xining 810007,China;Qinghai Provincial Drug Evaluation and Verification Center,Xining 810007,China;S&P Pharmaceutical Co.,Ltd.,Xining 810016,China)

机构地区：[1]西宁市食品药品检验检测中心,西宁810007 [2]青海省药品审评核查中心,西宁810007 [3]三普药业有限公司,西宁810016

出　　处：《药物分析杂志》2023年第2期209-218,共10页Chinese Journal of Pharmaceutical Analysis

基　　金：青海省基础研究项目(2020-ZJ-747)。

摘　　要：目的:建立抱茎獐牙菜指纹图谱及多指标成分含量测定方法,为其质量控制及评价提供参考。方法:采用Waters Sun Fir ODS C18色谱柱,甲醇-0.05%磷酸溶液为流动相梯度洗脱,体积流量为1.0 m L·min^(-1),检测波长为254 nm,柱温为30℃;建立高效液相色谱(HPLC)图谱,采用Chempattern化学计量学软件进行相似度分析。进一步通过HPLC法,测定样品中10个指标成分的含量,以寻找样品间的质量差异成分。结果:建立了抱茎獐牙菜HPLC指纹图谱,相似度均>0.904,并确认29个共有峰,指认出马钱苷酸、獐牙菜苦苷、龙胆苦苷、当药苷、芒果苷、异荭草素、苦龙胆酯苷、当药醇苷、1,8-二羟基-3,7-二甲氧基[口山]酮、雏菊叶龙胆酮10个色谱峰。含量测定显示,10个成分线性关系良好(r≥0.9993),加样回收率均在96.8%~99.3%,RSD<2.1%,平均含量分别为0.16%、3.63%、0.23%、0.38%、0.24%、0.26%、0.07%、1.13%、0.08%、0.25%。测定结果表明獐牙菜苦苷、当药醇苷等成分含量较高。结论:HPLC指纹图谱结合多指标成分含量测定方法适用于抱茎獐牙菜的质量控制与评价,该方法快捷简便、稳定可靠,可为建立藏药抱茎獐牙菜质量标准提供依据。Objective:To establish a method for simultaneous determination of fingerprint and multi-target ingredients and quality control and evaluation in Swertia franchetiana H.Smith by HPLC.Methods:The analysis was performed on Waters Sunfire ODS-C18column with mobile phase consisting of methanol-0.05%phosphoric acid solution with gradient elution at 1.0 mL·min^(-1).The detection wavelength was 254 nm and the columntemperature was 30℃.Samilarity evaluation combined with Chempattern chemical software was used to evaluate the fingerprint established by HPLC.The content of 10 index components were determined by HPLC.Results:Swertia franchetiana H.Smith fingerprint was established,similarity>0.904,29 common peaks were confirmed.10 chromatographic peaks of loganin,swertiamarin,gentiopicroside,sweroside,mangiferin,homoorientin,amarogentin,mercaptosid,1,8-dihydroxy-3,7-dimethoxyxanketo,bellidifolin were identified,ten components showed good linear relationships(r≥0.9993).The recoveries were 96.8%-99.3%,RSD<2.1%.The average content determination result were 0.16%,3.63%,0.23%,0.38%,0.24%,0.26%,0.07%,1.13%,0.08%,0.25%.The results showed that the content of swertiamarin and mercaptosid was high.Conclusion:The HPLC fingerprint combined with the content determination method of multi index components is suitable for the quality control and evaluation of Swertia franchetiana H.Smith.This method is fast,simple,stable and reliable,and can provide a basis for establishing the quality standard of Tibetan medicine Swertia franchetiana H.Smith.

关 键 词：抱茎獐牙菜 高效液相色谱 指纹图谱 马钱苷酸 獐牙菜苦苷 龙胆苦苷 当药苷 芒果苷 异荭草素 苦龙胆酯苷 当药醇苷 1 8-二羟基-3 7-二甲氧基[口山]酮 雏菊叶龙胆酮 

分 类 号：R917[医药卫生—药物分析学]