基于HPLC-QAMS同时测定荷叶水提物中6个成分的含量  

Simultaneous determination of six components in aqueous extract of lotus leaf by HPLC-QAMS

作　　者：王旭芳 魏敬东 刘伦 张欣蕾 李捷 王四旺 WANG Xu-fang;WEI Jing-dong;LIU Lun;ZHANG Xin-lei;LI Jie;WANG Si-wang(Department of Life Sciences and Medicine,Northwestern University,Xi'an 710069,China;Department of Traditional Chinese Medicine and Natural Pharmacology,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]西北北大学生命科学与医学部,西安710069 [2]空军军医大学中药与天然药物学教研室,西安710032

出　　处：《药物分析杂志》2023年第2期227-235,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：陕西省“13115”科技创新工程工程技术研究中心组建计划项目(NO.S2018-ZC-GCZXXY-SF-0005);陕西生物医药重点实验室平台(NO.2018SZS41)。

摘　　要：目的:建立一测多评法(QAMS)同时测定荷叶水提取物中O-去甲荷叶碱、槲皮素-3-O-桑布双糖苷、金丝桃苷、异槲皮苷、槲皮素-3-O-葡萄糖醛酸苷、荷叶碱的含量。方法:色谱柱为Lamdo Stamsil C18(250 mm×4.6mm,5μm),流动相为0.1%磷酸水溶液-乙腈,梯度洗脱,流速为0.6 mL·min^(-1),检测波长为270 nm,柱温为25℃,进样量为5μL。以O-去甲荷叶碱为内参物,计算其他5个成分的相对校正因子,并测定含量。结果:O-去甲荷叶碱、槲皮素-3-O-桑布双糖苷、金丝桃苷、异槲皮苷、槲皮素-3-O-葡萄糖醛酸苷、荷叶碱分别在各自范围内线性关系良好(r≥0.9995),精密度、重复性、稳定性试验结果的RSD均<3.0%,平均加样回收率为94.6%~108.0%,RSD为0.95%~2.1%。QAMS法测得荷叶水提物中上述6个成分的含量分别为0.23、3.43、1.69、10.59、41.23、0.69 mg·g^(-1)。结论:建立的荷叶水提物中6个成分的含量测定方法可靠,可用于其质量控制及药效基础研究。Objective:To establish a method for the simultaneous determination of O-nornuciferine,quercetin-3-O-sycambinoside,hyperoside,isoquercitrin,quercetin-3-O-glucuronide,and nuciferine in water extract of lotus leaf.Methods:The column was Lamdo Stamsil C18(250 mm×4.6 mm,5μm),the mobile phase was 0.1%phosphoric acid aqueous solution-acetonitrile in gradient mode at a flow rate of 0.6 mL·min^(-1).The detective wavelength was set at 270 nm.The column temperature was 25℃and the injection volume was 5μL.The relative correction factors of the other five components to O-nornuciferine were calculated and their contents were determined.Results:The linear relationships of O-nornuciferine,quercetin-3-O-sambulide,hyperoside,isoquercitrin,quercetin-3-O-glucuronide,and nuciferine were good(r≥0.9995).The RSDs of precision,repeatability and stability tests were less than 3.0%.The average recovery was 94.6%-108.0%with RSDs within 0.95%-2.1%.The content of the six components in the water extract of lotus leaf was determined by QAMS was 0.23,3.43,1.69,10.59,41.23,0.69 mg·g^(-1).Conclusion:The established method for the determination of 6 components in the water extract of lotus leaf is reliable and can be used for its quality control and pharmacodynamics research.

关 键 词：荷叶 水提取物 荷叶碱 高效液相色谱 一测多评法 O-去甲荷叶碱 金丝桃苷 异槲皮苷 

分 类 号：R917[医药卫生—药物分析学]