银杏叶提取物对抑郁模型小鼠内侧前额叶皮质氧化应激及锥体神经元兴奋/抑制平衡的影响  

Effects of ginkgo biloba extract on oxidative stress and the balance of excitation/inhibition of pyramidal neurons in the medial prefrontal cortex of depressive model mice

作　　者：朱传安 张贤华 陈进东 夏玉平 黄志源 马增明 谢彬 谢彦颖 谢奕彬 李桂文 Zhu Chuanan;Zhang Xianhua;Chen Jindong;Xia Yuping;Huang Zhiyuan;Ma Zengming;Xie Bin;Xie Yanying;Xie Yibin;Li Guiwen(Department of Integrated Traditional Chinese and Western Medicine,Xiamen Xianyue Hospital,Xiamen 361012,China;Department of Mental Health Research,Xiamen Xianyue Hospital,Xiamen 361012,China;Physical Therapy Department,Xiamen Xianyue Hospital,Xiamen 361012,China)

机构地区：[1]厦门市仙岳医院中西医结合科,厦门361012 [2]厦门市仙岳医院精神卫生研究室,厦门361012 [3]厦门市仙岳医院物理治疗室,厦门361012

出　　处：《中华行为医学与脑科学杂志》2023年第3期210-217,共8页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：福建省第七批省级中医重点专科项目(闽卫中医函[2019]262号);福建省卫建委科研计划项目(2020GGB065)。

摘　　要：目的:探讨银杏叶提取物(ginkgo biloba extract,GBE)对慢性不可预见性温和性应激(chronic unpredictable mild stress,CUMS)所致抑郁模型小鼠内侧前额叶皮层(medial prefrontal cortex,mPFC)氧化应激及锥体神经元兴奋/抑制(excitatory/inhibitory,E/I)平衡的影响。方法:48只SPF级7周龄雄性C57BL/6J小鼠按照随机数字表法分为4组:对照+生理盐水组(CTRL+Veh组)、对照+GBE组(CTRL+GBE组)、模型+生理盐水组(CUMS+Veh组)、模型+GBE组(CUMS+GBE组),每组12只小鼠。CUMS+Veh组和CUMS+GBE组小鼠采用CUMS方法建立抑郁模型,造模期间CTRL+GBE组和CUMS+GBE组小鼠给予腹腔注射GBE(70 mg/kg,1次/d),CTRL+Veh组和CUMS+Veh组小鼠无应激、腹腔注射等体积0.9%氯化钠溶液。造模结束后采用糖水偏爱实验、强迫游泳实验、悬尾实验检测小鼠抑郁行为,采用旷场实验检测小鼠自主运动、探索能力、焦虑行为。采用分光光度计法检测mPFC超氧化物歧化酶(superoxide dismutase,SOD)与丙二醛(malondialdehyde,MDA)水平,全细胞记录检测神经元E/I平衡,包括自发性兴奋性突触后电流(spontaneous excitatory postsynaptic currents,sEPSC)与自发性抑制性突触后电流(spontaneous inhibitory postsynaptic currents,sIPSC)。采用SPSS 23.0进行数据管理和统计分析,所有数据采用双因素(是否给予GBE,是否给予CUMS造模,用GBE×CUMS表示)方差分析进行统计。结果:(1)行为学结果显示:小鼠旷场中央区活动时间及运动总距离、糖水偏爱率比较,GBE×CUMS交互作用显著(F=24.90,4.82,3.91,均P<0.05)。简单效应分析结果显示,CUMS+Veh组小鼠旷场中央区活动时间[(47.15±3.58)s]及运动总距离[(19.33±0.86)m]、糖水偏爱率[(59.11±8.79)%]均低于CTRL+Veh组[(61.55±2.49)s、(23.24±1.21)m、(84.02±7.45)%](均P<0.01),CUMS+GBE组[(56.51±3.53)s、(20.75±1.31)m、(70.80±11.79)%]均高于CUMS+Veh组(均P<0.05)。各组小鼠的强迫游泳不动时间和悬尾不动时间比较,GBE×CUMS交互作用显著(F=Objective To investigate the effect of ginkgo biloba extract(GBE)on oxidative stress in medial prefrontal cortex and excitatory/inhibitory balance of pyramidal neurons in chronic unpredictable mild stress(CUMS)-induced depressive model mice.Methods Totally 48 SPF grade 7-week-old male C57BL/6J mice were divided into 4 groups according to random number table method:control+saline group(CTRL+Veh),control+GBE group(CTRL+GBE),model+saline group(CUMS+Veh),model+GBE group(CUMS+GBE),with 12 mice in each group.Mice in CUMS+Veh group and CUMS+GBE group were established by CUMS method,and mice in CTRL+GBE group and CUMS+GBE group were intraperitoneally injected with GBE(70 mg/kg)once a day,and mice in CTRL+Veh group and CUMS+Veh group were injected intraperitoneally with 0.9%sodium chloride solution.Then,the sucrose preference test,forced swimming test(FST)and tail suspension test(TST)were performed to evaluate the depressive-like behavior of mice,and open field test(OFT)was performed to evaluate the autonomous locomotion and exploration ability and anxiety-like behavior.The content of malondialdehyde(MDA)and activity of superoxide dismutase(SOD)in mPFC were determined by ELISA.Spontaneous excitatory postsynaptic currents(sEPSC)and spontaneous inhibitory postsynaptic currents(sIPSC)were detected by whole-cell recording.SPSS 23.0 was used for data analysis and two-factor analysis of variance(whether to get GBE,whether to mold,show as GBE×CUMS)was used for statistical analysis.Results(1)Behavioral results:the the time spent in center and total distance of OFT and sugar preference rate of the four groups of mice were compared,and the interaction of GBE×CUMS was significant(F=24.90,4.82,3.91,all P<0.05).The results of simple effect analysis showed that the time spent in center((47.15±3.58)s),the total distance((19.33±0.86)m)and the sugar preference rate((59.11±8.79)%)of the mice in CUMS+Veh group were lower than those in the CTRL+Veh group((61.55±2.49)s,(23.24±1.21)m,(84.02±7.45)%)(all P<0.01),and the above indexes in

关 键 词：抑郁 慢性不可预见性温和性应激 氧化应激 自发性兴奋性突触后电流 自发性抑制性突触后电流 小鼠 

分 类 号：R285.5[医药卫生—中药学]