高粱泡叶中毛蕊花糖苷的TLC鉴别和HPLC含量测定  

TLC Identification and HPLC Determination of Verbascoside in Rubus Lambertianus Ser Leaves

作　　者：田孟斌 谢朝宇 李旭东 吴静澜 郭兵 聂倩 TIAN Meng-bin;XIE Chao-yu;LI Xu-dong;WU Jing-lan;GUO Bing;NIE Qian(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China;National Engineering Research Center of Miao Medicine,Guiyang 550025,Guizhou,China;Guizhou Engineering Research Center of Traditional Chinese Medicine Processing and Preparation,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]国家苗药工程技术研究中心,贵州贵阳550025 [3]贵州中药炮制与制剂工程技术研究中心,贵州贵阳550025

出　　处：《贵州中医药大学学报》2023年第2期26-30,100,共6页Journal of Guizhou University of Traditional Chinese Medicine

基　　金：贵州省中医药管理局中医药、民族医药科学技术课题,项目编号:QZYY-2022-011;贵州中医药大学药用高分子材料研究中心,项目编号:贵中医党办发[2019]70;贵州省高等学校中药民族药(苗药)新剂型新制剂工程研究中心,项目编号:黔教技[2022]022。

摘　　要：目的:以毛蕊花糖苷为指标成分,建立高粱泡叶中的毛蕊花糖苷TLC鉴别和HPLC含量测定的方法。方法:使用TLC鉴别高粱泡叶中的毛蕊花糖苷成分;使用HPLC测定高粱泡叶中毛蕊花糖苷的含量。结果:TLC鉴别:以聚酰胺薄膜为薄层板,乙酸乙酯-甲苯-甲酸-甲醇(10:1.5:1:4)为展开剂,3%三氯化铝乙醇溶液为显色剂,置紫外光灯(365 nm)检视,所得薄层色谱分离效果和显色效果佳,斑点清晰。HPLC含量测定:色谱柱为WondaSilC18柱(4.6 mm×250 mm,5μm),流动相:2%磷酸水溶液(A)-乙腈(B),梯度洗脱,检测波长330 nm,柱温30℃,流速1.0 mL/min,进样10μL,结果表明毛蕊花糖苷在0.2368~0.4736μg线性关系良好(R=0.9997),加样回收率为98.5%,RSD为2.70%。结论:通过建立高粱泡叶中毛蕊花糖苷TLC鉴别和HPLC含量测定的方法,能够准确定性和定量分析高粱泡叶中毛蕊花糖苷,该方法操作简便快速,稳定可靠,专属性强,为其质量标准研究提供参考,也为其后续开发提供理论依据。Objective:To establish TLC identification and HPLC determination of verbascoside in Rubus Lambertianus Ser leaves by using verbascoside as the index component.Methods:The verbascoside components in Rubus Lambertianus Ser leaves were identified by TLC.HPLC was used to determine the content of verbascoside in Rubus Lambertianus Ser leaves.Results:TLC identification:To inspect under UV lamp(365 nm)by using polyamide film as a thin layer plate,ethyl acetate-toluene-formic acid-methanol(10:1.5:1:4)as the development agent,3%aluminum trichloride ethanol solution as the color development agent,the thin layer chromatography separation effect and color development effect were good,and the spots were clear.HPLC content determination:WondaSilC18 column(4.6 mm×250 mm,5μm),mobile phase:2%phosphoric acid solution(A)-acetonitrile(B),gradient elution,detection wavelength 330 nm,column temperature 30℃,flow rate 1.0 mL/min,injection of 10μL.The results showed that verbascoside had a good linear relationship between 0.2368~0.4736μg(R=0.9997),and the average recovery rate was 98.5%.The RSD was 2.70%.Conclusion:The accurate qualitative and uantitative analysis of verbascoside can be achieved by establishing the methods of TLC identification and HPLC determination of Verbascoside from Rubus Lambertianus Ser leaves.This method is simple,rapid,stable,reliable and specific,which provides a reference for the quality standard research and theoretical basis for its subsequent development.

关 键 词：高粱泡叶 毛蕊花糖苷 薄层色谱法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]