进境木材变孔异担子菌的KASP检测方法的建立  

Establishment of a KASP detection method for Heterobasidion irregulare on imported wood

作　　者：许剑涛 刘晓宇 钱茱希 郭静 杨静 李彬 吴翠萍 Xu Jiantao;Liu Xiaoyu;Qian Zhuxi;Guo Jing;Yang Jing;Li Bin;Wu Cuiping(Animal,Plant and Food Inspection Center,Nanjing Customs District,Nanjing 210001,China)

机构地区：[1]南京海关动植物与食品检测中心,江苏南京210001

出　　处：《植物检疫》2023年第2期55-61,共7页Plant Quarantine

基　　金：海关总署科研项目(2020HK159)。

摘　　要：变孔异担子菌(Heterobasidion irregulare)是一种侵染性强、危害大的针叶树病原菌。为建立变孔异担子菌的KASP检测方法,本研究根据变孔异担子菌GAPDH基因的SNP位点,筛选出变孔异担子菌KASP分子标记,并检测了该标记的特异性、灵敏度。结果表明,引物Hirr-FAM-F1、Hirr-HEX-F2、Hirr-R可以准确鉴定变孔异担子菌,检测灵敏度可达0.5 ng/μL。Heterobasidion irregulare is a fungus with strong infection and great hazard on coniferous wood.In order to establish a KASP detection method for H.irregulare,this study screened out a molecular marker of H.irregulare based on the SNP site of the GAPDH gene,and the specificity and sensitivity were tested.The results showed that Hirr-FAM-F1、Hirr-HEX-F2、Hirr-R could accurately identify H.irregulare and the detection sensitivity was up to 0.5 ng/μL.

关 键 词：变孔异担子菌 KASP标记 检测 

分 类 号：S41-34[农业科学—植物保护]